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首页> 外文期刊>Infection and immunity >Cellular defense of the avian respiratory system: influx and nonopsonic phagocytosis by respiratory phagocytes activated by Pasteurella multocida.
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Cellular defense of the avian respiratory system: influx and nonopsonic phagocytosis by respiratory phagocytes activated by Pasteurella multocida.

机译：禽呼吸系统的细胞防御：多杀性巴斯德氏菌激活的呼吸吞噬细胞的流入和非调理吞噬作用。

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Poultry have a very limited number of resident macrophages in the normal steady-state respiratory tract. Thus, poultry must rely heavily on active migration of phagocytic cells to the lungs and air sacs in defending against respiratory pathogens. Intratracheal administration of a live, apathogenic Pasteurella multocida vaccine (Choloral; Clemson University strain) increased the number of avian respiratory phagocytes (ARP; obtained by lavage of lungs and air sacs) within 24 h by 3 orders of magnitude compared with the number of ARP obtained from mock-inoculated controls and from nonreacting chickens. Chickens yielding a high number of ARP did not show any sign of respiratory disease. Flow cytometric analysis of ARP that were exposed to 20 nonopsonized fluorescent microspheres per ARP for 30 min at 37 degrees C demonstrated a fivefold increase in the percentage of actively phagocytic cells in the ARP populations of stimulated chickens compared with the percentage of phagocytic ARP for mock-inoculated control birds. The phagocytic capacity (relative number of engulfed microspheres) of ARP from stimulated birds doubled during the same time. The flow cytometric observations were confirmed by fluorescence microscopy. These results indicate that activation by avirulent replicating agents of phagocytic cells of chicken to migrate to the respiratory tract may be a means of defending poultry against air sacculitis and pneumonia.
机译：家禽在正常稳态呼吸道中的常驻巨噬细胞数量非常有限。因此，家禽在防御呼吸道病原体时必须在很大程度上依赖吞噬细胞向肺和气囊的迁移。经气管内施用活的，无病原性的多杀巴斯德氏菌疫苗（Choloral;克莱姆森大学株），与ARP数量相比，在24小时内使鸟类呼吸吞噬细胞（ARP;通过洗肺和囊囊获得）数量增加了3个数量级。从接种模拟疫苗的对照和未反应的鸡中获得。产生大量ARP的鸡没有任何呼吸道疾病迹象。在ARP流式细胞仪分析中，在ARP暴露于37°C的条件下，每个ARP暴露于20个非调理的荧光微球中30分钟，结果显示，与模拟的吞噬ARP的百分比相比，受激鸡的ARP种群中吞噬细胞的活跃吞噬细胞的百分比增加了五倍接种对照鸟类。在同一时间，来自受激鸟类的ARP的吞噬能力（吞噬的微球的相对数量）增加了一倍。通过荧光显微镜证实了流式细胞术观察。这些结果表明，无毒复制剂激活鸡的吞噬细胞迁移到呼吸道可能是保护家禽免于遭受空气囊炎和肺炎的一种手段。

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