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首页> 外文期刊>Tropical Animal Health and Production >Preliminary Studies by ELISA on the Antigen and Antibody Dynamics in the Early Stages of Experimental Infections with Trypanosoma evansi in Cattle
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Preliminary Studies by ELISA on the Antigen and Antibody Dynamics in the Early Stages of Experimental Infections with Trypanosoma evansi in Cattle

机译：ELISA法对牛埃文氏锥虫实验性感染早期抗原和抗体动力学的初步研究

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摘要

Six 6-month-old bulls were experimentally infected with five different isolates of Trypanosoma evansi; two received the same isolate and the other four received different isolates. The parasitaemias and serum antigen levels were monitored regularly by the haematocrit centrifuge technique (HCT) and antigen-detection ELISA (Ag-ELISA), respectively. Trypanosomal antigen was demonstrated by the Ag-ELISA by 10–14 days post inoculation in four cattle, while parasitaemias were first found to be positive in individual cattle over a longer period of time post inoculation (6–28 days). In two cattle, the Ag-ELISA values were also positive when the animals were found to harbour trypanosomes by the HCT and only turned negative 3 days after treatment, while the ELISA values fluctuated during the experiment in another two bulls. The remaining two cattle never produced positive ELISA results despite positive parasitological results. The antibody titres in all six cattle started to rise around 10 days post inoculation and then stayed high throughout the experiment. It was concluded that the Ag-ELISA would produce some false negative results in the early stages of T. evansi infection owing to variations in the balance of parasitaemia and antibody levels in the circulation, and in the pathogenicity of parasite strains.
机译：实验上对六只6个月大的公牛感染了五种伊万锥虫分离株。其中两个接受相同的隔离株，其他四个接受不同的隔离株。分别通过血细胞比容离心技术（HCT）和抗原检测ELISA（Ag-ELISA）定期监测寄生虫血症和血清抗原水平。在四只牛接种后10–14天通过Ag-ELISA证实了锥虫抗原，而首次在接种后较长时间（6–28天）中发现寄生虫血症呈阳性。在两只牛中，当通过HCT发现动物带有锥虫时，Ag-ELISA值也呈阳性，并且在治疗后3天才变为阴性，而在另外两只公牛的实验过程中，ELISA值则波动。尽管寄生虫学结果阳性，其余的两只牛从未产生过阳性的ELISA结果。接种后约10天，所有六头牛的抗体滴度开始上升，然后在整个实验中保持较高水平。结论是，由于寄生虫血症和循环中抗体水平的平衡以及寄生虫菌株的致病性变化，Ag-ELISA在伊文氏锥虫感染的早期会产生一些假阴性结果。

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