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首页> 外文期刊>Virchows Archiv >Immunohistochemical study on caveolin-1α in regenerating process of tubular cells in gentamicin-induced acute tubular injury in rats
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Immunohistochemical study on caveolin-1α in regenerating process of tubular cells in gentamicin-induced acute tubular injury in rats

机译：庆大霉素致大鼠急性肾小管损伤中肾小管-1α在肾小管细胞再生过程中的免疫组织化学研究

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摘要

Caveolin-1, a principal component of caveolae, modulates growth signaling, endocytosis, and intracellular transport. We examined the expression of caveolin-1α and its relation to cell cycle and caveolin-interacting growth factor receptors in regenerating proximal tubules (PTs) after gentamicin-induced acute renal failure in rats. Caveolin-1α appeared in regenerating PTs as early as day 4 after last gentamicin, peaked at days 6 to 8, and showed cytoplasmic pattern after day 8. Immunoelectron microscopy revealed caveolin-1α-positive caveolae on the cell membrane and in cytoplasms in regenerating PTs at days 4 to 8 and caveolin-positivity confined to cytoplasms after day 10. The number of PT cells with proliferation markers peaked at day 6 and decreased afterwards as expression of cyclin-dependent kinase inhibitors increased. Platelet-derived growth factor receptor-β (PDGFR-β) and epidermal growth factor receptor (EGFR) were colocalized with caveolin-1α in proliferating PTs as early as day 4. Phosphorylated EGFR increased at day 8 and afterwards when caveolins dissociated from EGFR or decreased. In case of PDGFR-β, phosphorylation seemed to be associated with the increase and association of caveolins to the receptors. Our results suggest that transient expression of caveolin-1α in early regenerating PTs might contribute to the regenerating process of PTs through modulating growth factor receptors.
机译：小窝蛋白1，小窝蛋白的主要成分，调节生长信号，内吞作用和细胞内转运。我们检查了庆大霉素诱导的大鼠急性肾衰竭后再生近端小管（PT）中caveolin-1α的表达及其与细胞周期和caveolin相互作用生长因子受体的关系。 Caveolin-1α早在最后一种庆大霉素之后的第4天就出现在再生的PT中，在第6至8天达到峰值，并在第8天后呈胞质模式。免疫电子显微镜检查显示，再生的PTs的细胞膜和细胞质中Caveolin-1α阳性小窝。在第4至第8天，小窝蛋白的阳性反应仅限于第10天的细胞质。具有增殖标志物的PT细胞数量在第6天达到峰值，随后随着细胞周期蛋白依赖性激酶抑制剂表达的增加而减少。血小板衍生的生长因子受体-β（PDGFR-β）和表皮生长因子受体（EGFR）早在第4天就与caveolin-1α在增殖的PT中共定位。磷酸化的EGFR在第8天开始升高，此后，当caveolins从EGFR分离或随后解离。减少。对于PDGFR-β，磷酸化似乎与小窝蛋白与受体的增加和缔合有关。我们的结果表明，caveolin-1α在早期再生PT中的瞬时表达可能通过调节生长因子受体促进PT的再生过程。

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  • 来源
    《Virchows Archiv》 |2007年第6期|671-681|共11页
  • 作者

    Yoshihide Fujigaki; Masanori Sakakima; Yuan Sun; Tetsuo Goto; Naro Ohashi; Hirotaka Fukasawa; Takayuki Tsuji; Tatsuo Yamamoto; Akira Hishida;

  • 作者单位

    First Department of Medicine Hamamatsu University School of Medicine 1-20-1 Handayama 431-3192 Hamamatsu Japan;

    First Department of Medicine Hamamatsu University School of Medicine 1-20-1 Handayama 431-3192 Hamamatsu Japan;

    First Department of Medicine Hamamatsu University School of Medicine 1-20-1 Handayama 431-3192 Hamamatsu Japan;

    First Department of Medicine Hamamatsu University School of Medicine 1-20-1 Handayama 431-3192 Hamamatsu Japan;

    First Department of Medicine Hamamatsu University School of Medicine 1-20-1 Handayama 431-3192 Hamamatsu Japan;

    First Department of Medicine Hamamatsu University School of Medicine 1-20-1 Handayama 431-3192 Hamamatsu Japan;

    First Department of Medicine Hamamatsu University School of Medicine 1-20-1 Handayama 431-3192 Hamamatsu Japan;

    First Department of Medicine Hamamatsu University School of Medicine 1-20-1 Handayama 431-3192 Hamamatsu Japan;

    First Department of Medicine Hamamatsu University School of Medicine 1-20-1 Handayama 431-3192 Hamamatsu Japan;

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  • 关键词

    Acute renal failure; Caveolae; Caveolin; Gentamicin; Proximal tubule; Regeneration; Platelet-derived growth factor; Epidermal growth factor;

    机译：急性肾衰竭;小窝;小窝蛋白;庆大霉素;近端小管;再生;血小板衍生的生长因子;表皮生长因子;

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