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首页> 外文期刊>Journal of Virology >Primary Virus-Cell Interactions in the Immuno-fluorescence Assay of Venezuelan Equine Encephalomyelitis Virus
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Primary Virus-Cell Interactions in the Immuno-fluorescence Assay of Venezuelan Equine Encephalomyelitis Virus

机译：委内瑞拉大当量的免疫荧光测定中的原发性病毒 - 细胞相互作用

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摘要

The conditions under which Venezuelan equine encephalomyelitis (VEE) virus attached to host cells markedly influenced the assay of virus by the fluorescent cell-counting technique. When virus inoculum was centrifuged onto McCoy cell monolayers, approximately 97% of virus was attached to cells within 10 min, in contrast to 34% after stationary incubation at 35 C for 2 hr. Maximal binding of virus occurred only in the presence of 0.1 to 0.15 m NaCl. This salt requirement, added to evidence of pH dependence and temperature independence of VEE virus attachment to cells, indicated that the initial union involved electrostatic forces. Virus penetration, measured by the insensitivity of virus-cell complexes to viral antiserum, was complete in 30 min at 35 C. The process was temperature-dependent and un-affected by the ionic content of medium. For assay of VEE virus by the fluorescent cell-counting technique, infected cells may be enumerated as early as 12 hr after infection of cell monolayers. The relationship between virus concentration and cell-infecting units was linear; the distribution of fluorescent cells was random. The virus assay was equivalent in sensitivity but more precise and rapid than that of intracerebral inoculation of mice.
机译：附着于宿主细胞的委内瑞拉大当量病毒的条件显着影响了荧光细胞计数技术对病毒的测定。当病毒接种物离心在McCoy细胞单层上时，约97％的病毒在10分钟内与细胞连接，相反，在35℃的固定孵育后2小时达到34％。病毒的最大结合仅在0.1至0.15m NaCl存在下发生。该盐要求添加到 p h依赖性和温度独立于小区的依赖性和温度独立性的证据中，表明初始联盟涉及静电力。通过病毒细胞复合物对病毒抗血清的不敏感性测量的病毒渗透，在35℃下在30分钟内完成。该方法是温度依赖性和受培养基离子含量的未受影响的。对于通过荧光细胞计数技术的VEE病毒的测定，可以早在细胞单层感染后12小时枚举感染的细胞。病毒浓度与细胞感染单位之间的关系是线性的;荧光细胞的分布是随机的。病毒测定相当于敏感性，但比对小鼠的脑接种更精确和快速。

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