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首页> 外文期刊>Journal of Virology >Characterization of the Low-Molecular-Weight RNAs Associated with the 70S RNA of Rous Sarcoma Virus
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Characterization of the Low-Molecular-Weight RNAs Associated with the 70S RNA of Rous Sarcoma Virus

机译：与肉瘤病毒70s RNA相关的低分子量RNA的表征

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Two low-molecular-weight RNAs are associated with the 70S RNA complex of Rous sarcoma virus: a previously described 4S RNA and a newly identified 5S RNA. The 4S RNA constitutes 3 to 4% of the 70S RNA complex or the equivalent of 12 to 20 molecules per 70S RNA. It exhibits a number of structural properties characteristic of transfer RNA as revealed by two-dimensional electrophoresis of oligonucleotides obtained from a T1 ribonuclease digest of the 4S RNA species. The 5S RNA is approximately 120 nucleotides in length, constitutes 1% of the 70S RNA complex or the equivalent of 3 to 4 molecules per molecules of 70S RNA, and is identical in nucleotide composition and structure to 5S RNA from uninfected chicken embryo fibroblasts. Melting studies indicate that the 5S RNA is released from the 70S RNA complex at the same temperature required to dissociate 70S RNA into its constituent 35S subunits. In contrast, greater than 80% of the 4S RNA is released from 70S RNA prior to its conversion into subunits. The possible biological significance of these 70S-associated RNAs is discussed.
机译：两个低分子量RNA与Rous Sarcoma病毒的70 S RNA复合物相关：先前描述的4 S RNA和新鉴定的5 S < / Em> RNA。 4 S RNA构成70 S RNA复合物的3至4％，或者每70℃/ em> RNA的相当于12至20分子。它表现出多种转移RNA的结构特性，如由4 S RNA物种的T1核糖核酸酶消化所获得的二维电泳所揭示的二维电泳。 5 S RNA的长度约为120个核苷酸，占70 S RNA复合物的1％或每分子为70 S的3至4分子 RNA，在核苷酸组合物中与来自未感染的鸡胚成纤维细胞的核苷酸组合物和结构相同，与5- em> s RNA相同。熔化研究表明，在将70 S RNA分离成其成分的相同温度下，将5 S RNA从70 S RNA释放35 S 亚基。相反，在转化为亚基之前​​，从70 S RNA中释放出40％的80％。讨论了这些70 S 的可能生物学意义。

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