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首页> 外文期刊>Journal of Virology >Properties of Noninfectious and Transforming Viruses Released by Murine Sarcoma Virus-Induced Hamster Tumor Cells
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Properties of Noninfectious and Transforming Viruses Released by Murine Sarcoma Virus-Induced Hamster Tumor Cells

机译：鼠肉瘤病毒诱导的仓鼠肿瘤细胞释放的非染势和转化病毒的性质

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The cell culture lines HTG2 and HTG3 were established from a transplantable hamster tumor induced by a murine sarcoma virus (strain Gz-MSV) after 17 and 60 in vivo passages, respectively. The viruses released by these two cell lines markedly differ in morphology, antigenic composition, infectivity, transforming ability, and enzymatic activity. HTG2 virions contained the sarcoma genome but were noninfectious for mouse and hamster cells (S+H-virus). HTG3 virions transformed hamster but not mouse cells. Whereas HTG2 cells and its virus contained murine type C virus gs-1 antigen, all HTG3 clonal lines expressed both murine and hamster type C virus gs-1 antigens. The RNA-dependent DNA polymerase activity of HTG2 virus was very low, whereas that of HTG3 virus was relatively high. HTG2 virions contained electron-lucent centers only. HTG3 virus consisted of the expected mixture of virions with electron-dense and electron-lucent centers. Many broken or incomplete virions were present in both viruses. HTG2 virus is a noninfectious “defective” sarcoma virus without detectable helper virus. Data obtained in these experiments suggest that HTG3 virus is a hamster type C virus pseudotype of Gz-MSV (Gz-MSV [HaLV]). The genome of Gz-MSV is capable of antigenic expression in heterologous cells and in the presence of heterologous viruses. Attempts to chemically activate hamster type C virus (HaLV) from HTG2 cells were unsuccessful. The HTG1 cell culture line, established from another Gz-MSV-induced hamster tumor, initially released a virus indistinguishable from the HTG2 virus. After in vitro passage, spontaneous activation of HaLV occurred in HTG1 cells, and the resultant Gz-MSV (HaLV) had properties similar to those of the HTG3 virus.
机译：细胞培养线HTG2和HTG3分别由在17和60后的鼠肉瘤病毒（菌株GZ-MSV）之后的可移植的仓鼠肿瘤建立。这两种细胞系释放的病毒在形态，抗原组合物，感染性，转化能力和酶活性方面显着不同。 HTG2病毒群体含有肉瘤基因组，但对小鼠和仓鼠细胞（S + H病毒）无排感。 HTG3病毒群岛转化了仓鼠但不是小鼠细胞。虽然HTG2细胞及其病毒含有鼠型C病毒GS-1抗原，但所有HTG3克隆都表达了鼠和仓鼠型病毒GS-1抗原。 HTG2病毒的RNA依赖性DNA聚合酶活性非常低，而HTG3病毒的RG3病毒相对较高。 HTG2病毒群体仅包含电子朗讯中心。 HTG3病毒由带有电子 - 密集和电子朗讯中心的病毒粒子的预期混合物组成。两种病毒存在许多破碎或不完全的病毒群体。 HTG2病毒是一种无染色的“缺陷”肉瘤病毒，没有可检测的辅助病毒。在这些实验中获得的数据表明HTG3病毒是GZ-MSV（GZ-MSV [Halv]）的仓鼠型C病毒假型。 GZ-MSV的基因组能够在异源细胞和异源病毒存在下进行抗原性表达。尝试从HTG2细胞中化学激活仓鼠类型C病毒（Halv）是不成功的。从另一个GZ-MSV诱导的仓鼠肿瘤建立的HTG1细胞培养线最初释放了与HTG2病毒无法区分的病毒。在体外通过后，HTG1细胞中发生HALV的自发激活，并且所得GZ-MSV（HALV）具有与HTG3病毒类似的性质。

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