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首页> 外文期刊>Journal of Virology >RNA-Dependent RNA Polymerase in Nuclei of Cells Infected with Influenza Virus
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RNA-Dependent RNA Polymerase in Nuclei of Cells Infected with Influenza Virus

机译：用流感病毒感染细胞细胞核中的RNA依赖性RNA聚合酶

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摘要

Nuclei purified from chicken embryo fibroblast cells infected with influenza (fowl plague) virus contain an RNA-dependent RNA polymerase. The in vitro activity of this enzyme is insensitive to actinomycin D, and is completely destroyed by preincubation with ribonuclease. Enzyme induction is prevented if cells are treated with actinomycin D or cycloheximide at the time of infection. RNA-dependent RNA polymerase activity increases rapidly in cell nuclei from 1 h postinfection, reaches a maximum at 3 to 4 h, then declines; a similar RNA polymerase activity in the microsomal cell fraction increases from 2 h postinfection and reaches a maximum at 5 to 6 h. The characteristics of the nuclear and microsomal enzymes in vitro are similar with respect to pH and divalent cation requirements. The in vitro products of enzyme activity present in the nuclear and microsomal fractions of cells infected for 3 and 5 h were characterized by sucrose density gradient analysis, and annealing to virion RNA. The microsomal RNA polymerase product contained 67 and 93% RNA complementary to virion RNA at 3 and 5 h, respectively; for the nuclear RNA polymerase product these values were 40% in each case.
机译：用培养基（禽瘟疫）病毒感染的鸡胚成纤维细胞细胞纯化的核含有RNA依赖性RNA聚合酶。该酶的体外活性对放线菌素D不敏感，并通过与核糖核酸酶预孵育完全破坏。如果在感染时用放射素D或环己酰亚胺处理细胞，则防止酶诱导。 RNA依赖性RNA聚合酶活性在1小时内迅速增加细胞核，在3至4小时达到最大值，然后下降;在微粒体细胞级分中的类似RNA聚合酶活性从2小时增加增加，并且在5至6小时达到最大值。核和微粒体酶的特征在于pH和二价阳离子要求类似。通过蔗糖密度梯度分析表征在感染3和5小时的细胞核和微粒体级分中存在的酶活性的体外产物，并向病毒肝RNA退火。微粒体RNA聚合酶产物分别含有67和93％的RNA，分别在3和5小时的5小时内互补的病毒菌RNA互补;对于核RNA聚合酶产物，每种情况下，这些值为40％。

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