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Cell-Free Translation of Paramyxovirus Messenger RNA

机译:Paramyxovirus Messenger RNA的无细胞翻译

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Polypeptides corresponding in electrophoretic mobility to virion polypeptides 1, 3, and 5 were made in a reticulocyte cell-free system to which 18S RNA from Sendai virus-infected cells was added. Immune precipitation was used to select relevant polypeptides from endogenous products. The cell-free product corresponding to virion polypeptide 3 (the nucleocapsid structure unit) was the most abundant; its tryptic peptides comigrated electrophoretically with tryptic peptides of polypeptide 3 isolated from virions. Other sedimenting classes of RNA from infected cells were tested; only the 28S fraction showed slight activity. Virion 50S RNA was inactive. These findings support the hypothesis that complementary RNA transcripts of paramyxovirion RNA are the templates for viral proteins.
机译:在电泳迁移率上对应于病毒虫多肽1,3和5的多肽,在网状细胞的无细胞系统中制备,加入来自仙台病毒感染细胞的18℃18℃。使用免疫沉淀来选择内源产品的相关多肽。对应于病毒虫多肽3(核衣壳结构单元)的无细胞产物是最丰富的;其胰蛋白酶肽用从病毒粒中分离的多肽3的胰蛋白酶肽电泳进行了电泳。测试来自感染细胞的其他沉积类RNA;只有28个 S 分数显示出轻微的活动。病毒赛50 S RNA无活性。这些发现支持的假设,即副XVirionRNA的互补RNA转录物是病毒蛋白的模板。

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  • 来源
    《Journal of Virology》 |1973年第5期|共8页
  • 作者

    D. W. Kingsbury;

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  • 收录信息 美国《科学引文索引》(SCI);
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