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首页> 外文期刊>Microbiology >Separation, Characteristics and Minimal Amino-Acid Requirements of Six Variants Derived from a Strain of Bacillus cereus
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Separation, Characteristics and Minimal Amino-Acid Requirements of Six Variants Derived from a Strain of Bacillus cereus

机译：六种变种的分离，特征和最小氨基酸要求来自芽孢杆菌菌株的六种变体

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SurmmaryMaintenance of a stock culture of Bacillus cereus by subculture on nutrient agar plates resulted in dissociation of the culture manifest as the development of fimbriate outgrowths from the initially entire peripheries of confluent streaks and isolated colonies. Auxanographic determination of minimal amino acid requirements of the dissociated culture indicated that it was composed of at least two types of organism: one was sensitive to (i.e. probably lysed by) L-lysine and the other resistant. They were further separated on the basis of sensitivity to lysine and colonial morphology into six different types. The microscopic appearance of organisms and their arrangement within colonies indicated that the original culture and each of the variants was a rough (R) type. At 28°, four variants required only L-glutamate for growth, while the remaining two required L-cysteine or L-α-alanine in addition. At 35° a greater number of amino acids had to be supplied for growth to occur. At neither temperature did the variants show a requirement for growth factors of the vitamin B-complex. These nutritional requirements, together with infrared spectra of whole organisms and biochemical characteristics determined by conventional methods, confirmed that each variant, though showing minor differences, did represent a culture of B. cereus. On auxanographic plates in the presence of L-lysine at 28°, three variants were lysed, one was unaffected, and the growth of two enhanced.
机译：通过营养琼脂转载的芽孢杆菌植物培养的SurmmaryMaintenainte植物培养基导致培养物的解离，作为从汇合条纹和分离的菌落的最初整个周边的抑制产物的发展。辅助测定解离培养物的最小氨基酸要求表明它由至少两种类型的生物组成：一种对（即，可能裂解）L-赖氨酸和其他抗性敏感。它们在对赖氨酸和殖民形态的敏感性的基础上进一步分离成六种不同类型。生物体的显微外观及其在菌落中的布置表明，原始培养和每个变体是粗糙（R）型。在28°处，4个变体仅需要L-谷氨酸的生长，而剩余的两种所需的L-半胱氨酸或L-α-丙氨酸。在35°处，必须提供更多数量的氨基酸以进行生长。在两个温度下，变体表明维生素B-复合物的生长因子的要求。这些营养需求与常规方法确定的全生物体的红外光谱和生化特征一起证实了每种变体，虽然表现出微小的差异，所以代表了B.培养物的培养物。在28°的L-赖氨酸存在下的辅助板上，裂解三种变体，一个不受影响，并且两种增强的生长。

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    《Microbiology》 |1965年第3期|共18页
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    W. B. MOORE;

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  • 收录信息 美国《科学引文索引》(SCI);
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