Effective biotransformation of Reactive Black 5 Dye Using Crude Protease from Bacillus Cereus Strain KM201428

机译：使用芽孢杆菌菌株粗蛋白酶菌株的反应性黑5染料的有效生物转化株菌株KM201428

获取原文

页面导航

摘要
著录项
相似文献
相关主题

摘要

Effective effluent treatment is a paramount step towards conserving the dwindling clean water resources.The present study describes the use of crude protease extract from Bacillus Cereus Strain KM201428 biotransformation of azo dye Reactive Black 5(RB5).Batch experimental results displayed over 97% decolorization efficiency with initial dye concentration of 1.0 x ICHM.The decolorization process was highly dependent on contact time,dye concentration and pH.The optimum contact time and pH for decolorization were 120 hours and pH 9 respectively at 25°C.Biotransformation of RB5 dye was monitored using UV-Vis spectrophotometer and formed metabolites characterized by LC-QTOF-MS.Comparison of resultant LC-QTOF-MS chromatograms after decolorization confirmed complete cleavage of RB5 dye.First order kinetic fitted well with experimental data for different RB5 dye concentrations.Lineweaver-Burk plot was used to describe the apparent relationship between the decolorization rate and the dye concentration at optimized condition with the coefficient of determination R2 =1.This study comprehensively illustrates the potential of crude protease from Bacillus Cereus Strain KM201428 as an effective and environmentally friendly bacterial isolate in biodegradation of RB5 and opens a new approach in the treatment of wastewaters contaminated with high load of azo dyes.

机译：有效的污水处理是节省Dwindling清洁水资源的迈索所迈出的迈出。本研究描述了从芽孢杆菌菌株的粗蛋白酶提取物中的粗蛋白酶提取物的使用，偶氮染料反应性黑5（RB5）的生物转化。特异性实验结果显示出超过97％的脱色效率初始染料浓度为1.0×Ichm。脱色过程高度依赖于接触时间，染料浓度和pH。最佳接触时间和pH分别为120小时，pH 9分别在25℃下进行测量的RB5染料。使用UV-VIS分光光度计和形成的代谢物，其特征在于LC-QTOF-MS.COMPARISON脱色后得到的LC-QTOF-MS色谱图，证实了RB5染料的完全切割。首次订购动力学与不同RB5染料浓度的实验数据.Linewer- Burk Plot用于描述脱色率和染料浓度之间的表观关系具有鉴定系数的优化条件R2 = 1.本研究综合地说明了芽孢杆菌菌株KM201428的粗蛋白酶作为RB5生物降解的有效和环保的细菌分离物的潜力，并在污染的废水处理中打开了一种新方法高负荷的偶氮染料。

著录项

来源
《International Conference on Technologies and Materials for Renewable Energy, Environment and Sustainability》|2019年|793-1567 p.|共10页
会议地点
作者
Wycliffe C.Wanyonyi; John M.Onyari; Paul M.Shiundu;
展开▼
作者单位

展开▼
会议组织
原文格式 PDF
正文语种
中图分类能源;
关键词
Biotransformation; Kinetic; Reactive Black 5 dye; Decolorization; Protease;

机译：生物转化;动力学;活性黑5 染料;脱色;蛋白酶;

相似文献

外文文献
中文文献
专利

1. Decolonization of azo dye reactive black B by Bacillus cereus strain HJ-1 [J] . Chien-Sen Liao, Chih-Hsin Hung, Sung-Lin Chao Chemosphere . 2013,第7期

机译：蜡状芽孢杆菌HJ-1脱偶氮染料活性黑B
2. Secondary cell wall polysaccharides of Bacillus anthracis are antigens that contain specific epitopes which cross-react with three pathogenic Bacillus cereus strains that caused severe disease, and other epitopes common to all the Bacillus cereus strains tested. [J] . Leoff C, Saile E, Rauvolfova J, Glycobiology. . 2009,第6期

机译：炭疽芽孢杆菌的次生细胞壁多糖是含有特定抗原决定簇的抗原，这些抗原决定簇与引起严重疾病的三种致病性蜡状芽孢杆菌菌株以及所有测试的蜡状芽孢杆菌菌株共有的其他表位交叉反应。
3. Secondary cell wall polysaccharides of Bacillus anthracis are antigens that contain specific epitopes which cross-react with three pathogenic Bacillus cereus strains that caused severe disease, and other epitopes common to all the Bacillus cereus strains tested [J] . Christine Leoff24 Elke Saile23 Jana Rauvolfova2 Conrad P Quinn3 Alex R Hoffmaster3 Wei Zhong2 Alok S Mehta2 Geert-Jan Boons2 Russell W Carlson12 and Elmar L Kannenberg24 Glycobiology . 2009,第6期

机译：炭疽芽孢杆菌的次生细胞壁多糖是含有特定抗原决定簇的抗原，这些抗原决定簇与引起严重疾病的三种致病性蜡状芽孢杆菌菌株以及所有测试的蜡状芽孢杆菌菌株共有的其他表位交叉反应。
4. Effective biotransformation of Reactive Black 5 Dye Using Crude Protease from Bacillus Cereus Strain KM201428 [C] . Wycliffe C.Wanyonyi, John M.Onyari, Paul M.Shiundu International Conference on Technologies and Materials for Renewable Energy, Environment and Sustainability . 2019

机译：使用芽孢杆菌菌株粗蛋白酶菌株的反应性黑5染料的有效生物转化株菌株KM201428
5. Patterns of reactivity of lantibiotics subtilin and nisin with molecular targets in Bacillus cereus and Bacillus subtilis 168. [D] . Kuntumalla, Srilatha. 2005

机译：蜡状芽孢杆菌和枯草芽孢杆菌168中羊毛硫抗生素枯草蛋白酶和乳酸链球菌素与分子靶标的反应模式。
6. Bacillus firmus strain FSS2C ameliorated oxidative stress in wheat plants induced by azo dye (reactive black-5) [O] . Faisal Mahmood, Muhammad Shahid, Sabir Hussain, 2020

机译：枯草芽孢杆菌菌株FSS2C在偶氮染料（反应性黑5）诱导的小麦植物中改善氧化应激
7. Production of moderately halotolerant, SDS stable alkaline protease from Bacillus cereus MTCC 6840 isolated from lake Nainital, Uttaranchal state, India Produção de protease alcalina moderadamente halotolerante e resistente a SDS, produzida por Bacillus cereus MTCC6840 isolado do lago Nainital, Estado de Uttaranchal, India [O] . Gopal K. Joshi, Sarvesh Kumar, Vinay Sharma 2007

机译：从印度北阿坎德邦奈尼塔尔湖分离的蜡状芽孢杆菌mTCC 6840产生中等耐盐性sDs稳定碱性蛋白酶生产从印度北安塔尔邦奈尼塔尔湖分离的蜡状芽孢杆菌mTCC6840产生的中度耐盐和sDs抗性碱性蛋白酶

Effective biotransformation of Reactive Black 5 Dye Using Crude Protease from Bacillus Cereus Strain KM201428

摘要

著录项

相似文献

相关主题

期刊订阅