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Carbohydrate Metabolism of Iron-Rich and Iron-Poor Staphylococcus aureus

机译:铁富含碳水化合物的碳水化合物和铁差的金黄色葡萄球菌

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SurmmaryComparative studies were made of the metabolic activities of a strain of Staphylococcus aureus grown in iron-poor and iron-rich trypticase medium, with and without glucose. Four nutritionally distinctive types of organisms were produced: iron-rich without glucose (Fe + G —); iron-poor without glucose (Fe— G —); iron-rich with glucose (Fe+ G +); iron-poor with glucose (Fe— G-). Fe+ G— cocci oxidized glucose, L- and D-lactate, pyruvate, acetate, formate, and Krebs cycle intermediates to completion. Of these substrates, only glucose and the lactates were oxidized by the Fe — G — organisms. These latter oxidations proceeded at decreased rates and incompletely to acetate and acetoin. Fe+ G + cocci in comparison with the Fe+ G— cocci showed glucose inhibition of oxidative capacity by metabolizing only glucose, L-lactate and pyruvate to acetate and failing to oxidize D-lactate, acetate and Krebs cycle intermediates. The Fe— G + cocci showed the most severe restriction of oxidative capacity by oxidizing only glucose with the accumulation of much lactate and minor amounts of pyruvate, acetate and acetoin. The Fe — G —, Fe — G +, and Fe + G + cocci glycolysed glucose at comparable rates, while the highly oxidative form Fe+ G— showed a markedly decreased glycolytic activity. The catalase activity of both types of iron-poor cocci was very much lower than that of the iron-rich organisms. Of the latter, the Fe + G+ cocci showed a catalase activity only 20% that of Fe + G — cocci.
机译:SurmmaryComprative研究是由铁贫菌和富含铁富含型胰蛋白酶培养基中生长的金黄色葡萄球菌菌株的代谢活性,有和没有葡萄糖。生产四种营养种类类型的生物:无葡萄糖的铁(Fe + G - );无葡萄糖(Fe-G - )的熨斗;富含葡萄糖(Fe + G +);葡萄糖(Fe-g-)的铁差。 Fe + G-Cocci氧化葡萄糖,L-和D-乳酸,丙酮酸,乙酸盐,甲酸和克雷斯循环中间体完成。在这些底物中,仅通过Fe - G - 生物氧化葡萄糖和乳酸盐。后一种氧化率下降,速率下降,对乙酸盐和丙酮酸不完全。与Fe + G-Cocci相比,Fe + G + COCC1通过仅代谢葡萄糖,L-乳酸和丙酮酸对乙酸盐并未氧化D-乳酸,醋酸盐和克雷布斯循环中间体,表现出葡萄糖抑制氧化能力。 Fe-g + Cocci通过仅氧化葡萄糖和少量丙酮酸,乙酸盐和丙酮蛋白的积累,仅通过仅氧化葡萄糖来表示最严重的氧化能力限制。在可比较速率下Fe - G - ,Fe - G +和Fe + G + Cocci糖酵解葡萄糖,而高氧化形式Fe + G-显示出显着降低的糖酵母活性。两种类型的铁可怜的COCCI的过氧化氢酶活性远低于铁富含生物体的。在后者中,Fe + G + Cocci显示过氧化氢酶活性仅为Fe + G - Cocci的20%。

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