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首页> 外文期刊>Journal of Virology >Specific Role of Each Human Leukocyte Type in Viral Infections II. Phytohemagglutinin-treated Lymphocytes as Host Cells for Vesicular Stomatitis Virus Replication In Vitro
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Specific Role of Each Human Leukocyte Type in Viral Infections II. Phytohemagglutinin-treated Lymphocytes as Host Cells for Vesicular Stomatitis Virus Replication In Vitro

机译：每种人白细胞类型在病毒感染中的特定作用II。 Phytohemagglutinin治疗淋巴细胞作为宿主细胞，用于体外囊泡口炎病毒复制

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摘要

The mitogenic agent, phytohemagglutinin (PHA), added to human mixed leukocyte cultures and to lymphocyte cultures converted small lymphocytes into lymphoblasts and increased lymphocyte susceptibility to vesicular stomatitis virus (VSV). Maximum virus yields were 30- to 1,000-fold higher in PHA-treated than in control cultures. VSV replicated to peak titers before lymphocytes were morphologically transformed by PHA, and virus titers fell as lymphoblast destruction began. PHA neither induced significant VSV replication in polymorphonuclear leukocyte cultures, nor increased the large virus yields in monocyte cultures. The treatment of PHA with heat, digestive enzymes, rabbit anti-PHA serum and serial dilutions failed to dissociate that portion of the PHA extract responsible for the conversion of lymphocytes into virus-susceptible cells from those components responsible for leukoagglutination or lymphocyte transformation.
机译：促霉素剂，植物混合白细胞培养物和淋巴细胞培养物中加入淋巴细胞的淋巴细胞和淋巴细胞易感性对淋巴细胞（VSV）增加。在对照培养物中，在PHA处理的最高病毒产率在PHA处理中较高30至1,000倍。在淋巴细胞形态学上由PHA改变淋巴细胞转化之前，VSV复制至峰值滴度，并且随着淋巴细胞破坏开始，病毒滴度下降。 PHA既不诱导多核白细胞培养物中的显着VSV复制，也没有增加单核细胞培养物中的大病毒产量。用热量，消化酶，兔抗PHA血清和连续稀释液处理PHA未能解离该部分，其负责将淋巴细胞转化成病毒易感细胞的PHA提取物，这些组分负责用于白细胞凝集或淋巴细胞转化的组分。

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