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Separation of a New Deoxyribonucleic Acid Polymerase from Vaccinia-infected HeLa Cells

机译:从痘苗病感染的HeLa细胞中分离新的脱氧核糖核酸聚合酶

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HeLa cell deoxyribonucleic acid (DNA) polymerase was purified about 100-fold by sequential column chromatography on phosphocellulose, hydroxylapatite, and Bio Rex 70. A new form of DNA polymerase found in vaccinia virus-infected cells was separated from HeLa DNA polymerase by chromatography on diethylamino-ethyl cellulose. The new form was also purified approximately 100-fold in the same manner as the HeLa DNA polymerase. In addition to chromatographic differences, the two enzymes differed with regard to primer response, relative activity at high pH, inactivation by heat and p-chloromercuribenzoate, and inhibition by vaccinia antiserum.
机译:通过序列粒子纤维素,羟基磷灰石和生物REX 70上的序列色谱法纯化HeLa细胞脱氧核糖核酸(DNA)聚合酶。通过色谱法从HeLa DNA聚合酶中分离出在痘苗病病毒感染细胞中发现的新形式的DNA聚合酶二乙基氨基 - 乙基纤维素。新形式也以与HeLa DNA聚合酶相同的方式纯化约100倍。除了色谱差异之外,两种酶在引物响应方面不同,高 P H的相对活性,通过热量和 P -Choromcribenzoate,以及痘苗病毒抗血清的抑制作用。

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