“L-cell virion,” obtained from chronically infected 929 L-cell lines, was purified on 70% sucrose pads and its ribonucleic acid (RNA) was extracted and analyzed on sucrose gradients. Two RNA components were isolated from the virion, a rapidly sedimenting species (82S) and a more slowly sedimenting species (6S). When the fast-moving component was heated to 45 C, it yielded a homogeneous peak which now sedimented at approximately 72S. Upon heating to 70 C, the peak dissociates into a smaller homogeneous RNA species with a sedimentation coefficient of approximately 33S. Ribonuclease treatment and base composition analysis revealed that the fast-moving species is a single-stranded molecule. Base ratio analysis also revealed that the small (6S) RNA component is similar in its base composition to L-cell ribosomal RNA and distinctly different from the base composition of the large (72S) RNA component. Actinomycin D inhibits production of the L-cell virion by the L-cell line.
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机译：从慢性感染的929L细胞系中获得的“L细胞病毒酮”在70％蔗糖垫上纯化，并在蔗糖梯度上萃取并分析其核糖核酸（RNA）。从病毒夫中分离出两种RNA成分，一种快速沉淀物种（82 S ）和更慢的沉淀物种（6 S ）。当快速移动的组分加热至45℃时，它产生了均匀的峰，该峰值现在在约72℃下沉淀。在加热至70℃时，峰值分离成较小的均质RNA物种，其沉降系数约为33 S 。核糖核酸酶处理和基础组成分析显示，快速移动的物种是单链分子。碱基比分析还显示，小（6 S ）RNA组分在其基础组合物中与L细胞核糖体RNA相似，与大的基础组合物明显不同（72 s < / EM>）RNA组分。放射素蛋白D抑制L细胞系L细胞病毒素的产生。
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