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首页> 外文期刊>Journal of Virology >Response of Simian Virus 40-Transformed Cell Lines and Cell Hybrids to Superinfection with Simian Virus 40 and Its Deoxyribonucleic Acid
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Response of Simian Virus 40-Transformed Cell Lines and Cell Hybrids to Superinfection with Simian Virus 40 and Its Deoxyribonucleic Acid

机译：Simian病毒40转化细胞系和细胞杂种与Simian病毒40的响应及其脱氧核糖酸的反应

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Whereas normal human and monkey cells were susceptible both to intact simian virus 40 (SV40) and to SV40 deoxyribonucleic acid (DNA), human and monkey cells transformed by SV40 were incapable of producing infectious virus after exposure to SV40, but displayed susceptibility to SV40 DNA. On the other hand, mouse and hamster cells, either normal or SV40-transformed, were resistant both to the virus and to SV40 DNA. Hybrids between permissive and nonpermissive parental cells revealed a complex response: whereas most hybrids tested were resistant, three of them produced a small amount of infectious virus upon challenge with SV40 DNA. All were resistant to whole virus challenge. The persistence of infectious SV40 DNA in permissive and nonpermissive cells up to 96 hr after infection was ascertained by cell fusion. The decay kinetics proved to be quite different in permissive and nonpermissive cells. Adsorption of SV40 varied widely among the different cell lines. Very low adsorption of SV40 was detected in nonsusceptible cells with the exception of the mKS-BU100 cell line. A strong increase in SV40 adsorption was produced by pretreating cells with polyoma virus. In spite of this increased adsorption, the resistance displayed by SV40-transformed cells to superinfection with the virus was maintained.
机译：虽然正常的人和猴细胞易于完整的Simian病毒40（SV40）和SV40脱氧核糖核酸（DNA），由SV40转化的人和猴细胞不能在暴露于SV40后产生传染性病毒，但显示对SV40 DNA的易感性。另一方面，鼠标和仓鼠细胞，正常或SV40转化，对病毒和SV40 DNA耐药。杂种和非智力亲本细胞之间的杂种揭示了复杂的反应：而大多数测试的杂交种是抗性的，而其中三种在攻击中产生少量的传染性病毒，而SV40 DNA攻击则产生少量的传染性病毒。一切都对整个病毒挑战抗性。通过细胞融合，确定感染后允许的传染性SV40 DNA在允许和非智能细胞中的持续性高达96小时。衰减动力学证明在允许和非智能细胞中是完全不同的。 SV40的吸附在不同的细胞系中广泛变化。除了MKS-Bu100细胞系外，在非批化细胞中检测到SV40的非常低的吸附。通过使用多瘤病毒预处理细胞产生SV40吸附的强烈增加。尽管对吸附增加了，但维持了SV40转化细胞显示对病毒的超蛋白的电阻。

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