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首页> 外文期刊>Journal of Virology >Immunological Basis of the Adenovirus 8-9 Cross-Reaction
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Immunological Basis of the Adenovirus 8-9 Cross-Reaction

机译：腺病毒8-9交叉反应的免疫基础

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The dedecon and hexon components of adenovirus types 8 and 9 have been extensively purified for use in establishing the basis of the cross-reaction between these types. Dodecons, the complete hemagglutinins, were purified 304- to 362-fold by fluorocarbon extraction, calcium phosphate batch chromatography, and ion-exchange column chromatography. Hexons, the group complement-fixation (CF) antigens, were purified 230- to 240-fold by erythrocyte adsorption, ion-exchange chromatography, and exclusion chromatography. Component antisera prepared in rabbits were tested in reciprocal fashion with crude virus and dodecon and hexon components. By hemagglutination-inhibition (HI), the dodecons of types 8 and 9 demonstrated the same predominantly one-sided relationship characteristic of the crude antigens. Some neutralizing activity was associated with both dodecons and hexons of each type. However, combining anti-dodecon and anti-hexon sera or producing antisera against the combined dodecon-hexon components resulted in neutralizing titers which were identical to titers obtained with antisera against the crude virus harvests. Dedecons of each type appear to share at least one antigenic determinant with hexons of the same type, and this determinant may reside on the vertex capsomere. Hexons possess group- and type-specific determinants, as shown by CF, neutralization, and immunodiffusion tests, and may exhibit some minor relationship between types 8 and 9. The results with the purified components are consistent with the predominantly one-sided antigenic relationship between types 8 and 9 in the conventional HI tests and the largely type-specific relationship by neutralization tests.
机译：腺病毒类型8和9的Dedecon和己酮组分被广泛纯化，以便在这些类型之间建立交叉反应的基础。十二烷，完整的血凝素，通过氟碳萃取，磷酸钙批选色和离子交换柱色谱纯化304至362倍。通过红细胞吸附，离子交换色谱和排阻色谱法纯化230至240倍的六己。群体补蛋白固定（CF）抗原。用粗糙的病毒和十二烷和十二烷组分以越野时尚进行兔子制备的成分抗血清。通过血聚凝集抑制（HI），第8型和9型的十二烷源证明了粗抗原的主要翼状关系特性。一些中和活性与每种类型的十二锭和己糖相关。然而，将抗十二烷酮和抗己顿血清组合或产生抗血清，其抵抗组合的十二烷 - 己酮组分导致中和滴度，其与用抗血清获得的滴度与粗ue病毒收获相同。每种类型的Dedecons似乎与相同类型的己酮共享至少一个抗原决定蛋白，并且该决定簇可能居住在顶点胶囊上。横向具有群体和类型特异性的决定因素，如CF，中和和免疫分离性试验所示，并且可能在8和9型之间表现出一些微小的关系。纯化组分的结果与主要的单面抗原关系一致传统的HI测试中的8和9种和通过中和测试的主要特定关系。

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