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Isolation and Characterization of Escherichia coli bacteriophage Ω 8 specific for E. coli strains belonging to sero-group O 8

机译:对属于血清基团的大肠杆菌菌株的大肠杆菌噬菌体ω8的分离与表征。

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SUMMARY: A bacteriophage which is specific for Escherichia coli O8 strains was isolated from Freiburg sewage. It is designated as ωOM 8. Inhibition studies suggested that the O8 specific lipopolysaccharide (isolated from E. coli O8) may be the phage receptor or part of it. Bacteriophage ωOM 8 also causes clearing on lawns of E. coli O93 if added at high multiplicity but it does not multiply in this strain. Escherichia coli strains O8 and O93 cross-react serologically and their (O antigenic) lipopolysaccharides have similar structures. Electron microscopy revealed the following features of ωOM 8: icosahedric head, 48 to 50 nm. in diameter; base plate consisting of 6 units, total diameter 14 nm.; 6 spikes, 12·0 to 14·5 nm. long and 3·5 to 4·0 nm. thick; 6 fibres, 168 nm. long, bent at 56 nm., 1·5 nm. thick. The propagation characteristics of ωOM 8 are: burst size, 130; latent period, 19 min.; eclipse period, 13 min.
机译:发明内容:从弗赖堡污水中分离出对大肠杆菌O8菌株的噬菌体。它被指定为ωom8.抑制研究表明O8特异性脂多糖(从大肠杆菌O8分离)可以是噬菌体受体或其中一部分。噬菌体ωom8还导致大肠杆菌O93的草坪,如果在高分子下添加,但在该应变中不常量。大肠杆菌Coli菌株O8和O93血清术和其(O抗原)脂多糖具有相似的结构。电子显微镜显示出以下特征ωom8:icosahedric head,48至50 nm。在直径上;基板由6个单位组成,总直径14nm。 6穗,12·0至14·5 nm。长3·5至4·0 nm。厚的; 6纤维,168nm。长,弯曲56纳米。,1·5纳米。厚的。 ωom8的传播特性是:突发大小,130;潜伏期,19分钟; Eclipse期间,13分钟。

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