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首页> 外文期刊>The biochemical journal >The oligosaccharide units of rabbit immunoglobulin G. Asymmetric attachment of C2-oligosaccharide
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The oligosaccharide units of rabbit immunoglobulin G. Asymmetric attachment of C2-oligosaccharide

机译：兔免疫球蛋白G.的寡糖单元G. C2-寡糖的不对称附着

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pTwo populations of immunoglobulin G (IgG) molecules were isolated from pooled rabbit serum. The first was almost devoid of galactosamine and was obtained in a yield of 7% of the total IgG; the second contained on average a single residue of galactosamine per molecule and was obtained in a yield of 30% of the total IgG. The galactosamine, which is present solely in the Ci2/i-oligosaccharide, appeared to be present on one H-chain and not the other in the four-chain structure. Evidence was obtained by the isolation of a glycopeptide linked through a disulphide bridge to a second peptide of the same sequence; the oligosaccharide attached to the first peptide was absent from the complementary peptide. Further evidence was obtained by degradation and analysis of the 5S IgG fragment, which comprises an intact half-molecule coupled through a disulphide bridge to the Fc fragment derived from the opposing half molecule (Goodman, 1965); only the intact H-chain carried the Ci2/i-oligosaccharide./p
机译：>从合并的兔血清中分离出两种免疫球蛋白G（IgG）分子的群体。第一个几乎没有半乳糖胺，得到了总IgG的7％的产率;第二个含有平均每种分子的半乳糖胺残基，并获得总IgG的30％的产率。本质上仅在C 2-o> - 醇糖中存在的半乳糖胺出现在一个H型链上，而不是四链结构中的另一个。通过将通过二硫桥连接到相同序列的第二肽的糖肽分离来获得证据。互补肽不存在附着在第一肽上的寡糖。通过降解和分析5S IgG片段的降解和分析，其包括通过二硫桥偶联的完整半分子与来自相对的半分子（Goodman，1965）的Fc片段偶联的完整半分子;只有完整的H-Chain携带C 2 - 醇酸糖。

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