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首页> 外文期刊>Journal of Virology >Detection and Assay of Avian Tumor Virus Group-Specific Antigen and Antibody by the Paired Radioiodine-Labeled Antibody Technique
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Detection and Assay of Avian Tumor Virus Group-Specific Antigen and Antibody by the Paired Radioiodine-Labeled Antibody Technique

机译：通过成对的放射性碘标记的抗体技术检测和测定禽肿瘤病毒组特异性抗原和抗体

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摘要

The paired radioiodine-labeled antibody technique (PRILAT) was applied to the detection and quantitation of avian tumor virus group-specific (gs) antigens and antibody. The technique proved to be specific, repeatable, and appreciably more sensitive than the microcomplement-fixation test for avian leukosis (COFAL). The PRILAT facilitated direct measurement of comparative antigen content of several types of transformed, neoplastic, or virus-infected cells and the magnitude of nonspecific antibody binding by appropriate control cells. The versatility of the technique was illustrated by application to the detection and quantitation of gs antibody content of chicken, turkey, pigeon, and hamster sera. Antibodies were detected in COFAL-negative sera from hamsters bearing tumors induced by the Schmidt-Ruppin strain of Rous sarcoma virus. Sera from chickens bearing similar tumors were not positive for gs antibodies, although sera from turkeys and chickens immunized with avian leukosis virus did contain gs antibodies.
机译：将配对的放射性碘标记的抗体技术（PRILAT）应用于禽肿瘤病毒组特异性（GS）抗原和抗体的检测和定量。该技术被证明是特定的，可重复的，并且比禽血管菌（Cofal）的微型固定试验更敏感。 Prilat促进了几种转化的，肿瘤或病毒感染细胞的比较抗原含量的直接测量，并通过适当的对照细胞结合的非特异性抗体的大小。通过应用于鸡，土耳其，鸽子和仓鼠血清的Gs抗体含量的检测和定量来说明该技术的多功能性。在患有由肉瘤病毒的Schmidt-ruppin菌株诱导的肿瘤患者的兔子中检测到抗体在Cofal阴性血清中检测到抗体。来自鸡的血清具有类似肿瘤的GS抗体对GS抗体不阳性，尽管用禽类失利病毒免疫的火鸡和鸡的血清含有GS抗体。

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