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Virus-Associated Nucleases: Evidence for Endonuclease and Exonuclease Activity in Rabbitpox and Vaccinia Viruses

机译:病毒相关的核酸酶:用于兔斑和痘苗病毒的内切核酸酶和外切核酸酶活性的证据

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The endonucleolytic action of a deoxyribonuclease activity in rabbitpox and vaccinia virus was established by change in sedimentation rate of denatured 3H-lambda deoxyribonucleic acid substrate. The presence of two deoxyribonuclease activities in pox-virus is confirmed. Exo- and endonuclease activities are unmasked by treatment of purified virus with the detergent Nonidet P-40 and further enhanced by treatment of viral “cores” with trypsin.
机译:通过变性 3 H-Lambda脱氧核糖核酸底酸基质的沉降速率的变化建立了脱氧氧基核糖核酸酶活性的脱氧氧基核酸酶活性的内核作用。确认了痘病毒中两种脱氧氧核酸酶活性的存在。通过用洗涤剂Nonidet P-40治疗纯化病毒,通过用胰蛋白酶治疗病毒“核”进一步增强,通过治疗纯化病毒,通过胰蛋白酶治疗病毒的病毒取消掩蔽。

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