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Adenosine 3',5'-cyclic monophosphate in Vibrio cholerae.

机译：腺苷3'，5'-循环单磷酸在慢性胆拉蛋白中。

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摘要

The extracellular concentration of cyclic adenosine 3',5'-monophosphate (AMP) of three different strains of Vibrio cholerae growing in syncase medium were measured. Cyclic AMP secreted by V. cholerae 569B varied widely, with different carbon sources. Mutant 13, which produced little or no toxin, released half the amount of cyclic AMP as the wild type. The release of less cyclic AMP into the medium by mutant 13 may be accounted for by the lower activity of adenylate cyclase observed. High glucose (3%) in the culture medium reduced the concentration of cyclic AMP both in wild type and mutant 13. Reduction of cyclic AMP levels at high concentrations of glucose (3%) occurred without change of adenylate cyclase activity. The release of enterotoxin to the medium varied with carbon sources but was independent of conditions which reduced the cyclic AMP both within the cell and the medium. Neither adenylate cyclase activity nor toxin production was reduced by an increase concentration of glucose in wild-type V. cholerae, whereas cyclic AMP levels were reduced by sixfold. A lower activity of the adenylate cyclase was observed in a mutant of V. cholerae which produced no detectable toxin. Thus, a correlation exists between toxin production and adenylate cyclase activity in V. cholerae.
机译：测定了三种不同菌株在同步培养基中生长的三种不同振动慢性菌株的环状腺苷3'，5'-单磷酸盐（AMP）的细胞外浓度。由V.Cholerae 569B分泌的循环放大器广泛变化，具有不同的碳源。产生很少或没有毒素的突变体13，作为野生型释放了循环放大器量的一半。通过突变体13释放较少的循环amp进入培养基中，可以通过观察到的腺苷酸环酶的较低活性来计算。培养基中的高葡萄糖（3％）降低了野生型和突变体13中的环形倍乳膏的浓度。在没有变化的腺苷酸环化酶活性的情况下，发生高浓度葡萄糖（3％）的循环AMP水平的循环AMP水平。肠毒素释放到培养基中随碳源而变化，但与细胞和培养基中的环状放大器的条件无关。通过野生型V.霍乱中的葡萄糖浓度增加，亚苯基酸酯环酶活性和毒素产生都不减少，而六倍循环AMP水平降低。在霍乱的突变体中观察到腺苷酸环酶的较低活性，其不含可检测的毒素。因此，在V.霍乱的毒素生产和腺苷酸环酶活性之间存在相关性。

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