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首页> 外文期刊>Infection and immunity >Partial purification and characterization of RTG-2 fish cell interferon.
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Partial purification and characterization of RTG-2 fish cell interferon.

机译：rTG-2鱼细胞干扰素的部分纯化与表征。

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摘要

Interferon produced by rainbow trout gonadal cells (RTG-2) was partially purified. The physical, chemical, and biological properties of this in vitro produced fish cell interferon were studied. Purification was achieved by ultracentrifugation, molecular sieve gel chromatography, ion exchange chromatography, and polyacrylamide gel electrophoresis. The isoelectric point of RTG-2 interferon, as determined by CM-Sephadex (C-50) chromatography, was 7.1. Filtration through Sephadex G-150 showed that RTG-2 interferon had a molecular weight of 94,000. The partially purified material was not sedimented at 105,000 times g for 2 h at 4 C. The fish cell interferon was non-dialyzable and exhibited heat and pH stability. The partially purified material was inactivated by treatment with trypsin or 2-mercaptoethanol, but was resistant to treatment with deoxyribonuclease or ribonuclease. RTG-2 interferon which was induced by infectious pancreatic necrosis virus exhibited antiviral activity against challenge with infectious hematopoietic necrosis virus or infectious pancreatic necrosis virus. Partially purified RTG-2 interferon exhibited greater species specificity than the crude material.
机译：部分纯化由虹鳟鱼ronadal细胞（RTG-2）产生的干扰素。研究了这种体外产生的鱼细胞干扰素的物理，化学和生物学性质。通过超速离心，分子筛凝胶色谱，离子交换色谱和聚丙烯酰胺凝胶电泳实现纯化。由CM-Sephadex（C-50）色谱法测定的RTG-2干扰素的等电点为7.1。通过Sephadex G-150过滤表明RTG-2干扰素的分子量为94,000。在4℃下，部分纯化的材料在105,000次G中未在105,000次G中沉淀。鱼细胞干扰素是不可透析的并且表现出热量和pH稳定性。通过用胰蛋白酶或2-巯基乙醇处理将部分纯化的材料灭活，但是用脱氧核酸脲或核糖核酸酶处理耐受处理。通过传染性胰腺坏死病毒诱导的RTG-2干扰素表现出抗病毒活性免受传染病造血坏死病毒或传染性胰腺坏死病毒的攻击。部分纯化的RTG-2干扰素表现出比粗物质更大的物种特异性。

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