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首页> 外文期刊>Journal of Virology >Structural proteins of simian virus 40. I. Histone characteristics of low-molecular-weight polypeptides.
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Structural proteins of simian virus 40. I. Histone characteristics of low-molecular-weight polypeptides.

机译：Simian病毒的结构蛋白40. I.低分子量多肽的组蛋白特征。

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摘要

The DNA-associated polypeptides of simian virus 40 (SV40), VP4 (mol wt 14,000), VP5 (mol wt 12,000), and VP6 (mol wt 11,000), have several properties characteristic of cell histones. After extraction from purified SV40 with dilute acids, these three polypeptides co-electrophoresed on low pH polyacrylamide gels with monkey-kidney cell histones F3, F2b, and F2a1. No virus polypeptide co-electrophoresed with histone F1. Polypeptides VP4, 5, and 6 lacked tryptophan, and only VP4 contained cysteine, as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis of virus labeled in vivo with (3H)lysine and either (14C)tryptophan or (35S)cystine. All of the capsid polypeptides VP1, 2, and 3 contained tryptophan whereas only VP1 and 2 contained cysteine. In addition, VP4, 5, and 6 are rich in arginine and lysine when compared with virus labeled with a mixture of amino acids. Analysis of virus grown in cells labeled prior to infection showed that VP4, 5 and 6 were labeled fivefold greater than the major capsid polypeptide, VP1, which indicates that they were partially derived from preexisting cell histones. Based on these data and on previously determined molecular weight estimates, we conclude that VP4, 5, and 6 are histones F3, F2b, and F2a1, respectively, although the possibility that SV40 contains a small amount of F2a2 could not be excluded.
机译：Simian病毒40（SV40），VP4（Mol WT 14,000），VP5（Mol WT 12,000）和VP6（Mol WT11,000）的DNA相关多肽具有细胞组蛋白的几种特征。在用稀酸中萃取纯化的SV40后，将这三种多肽在低pH聚丙烯酰胺凝胶上电泳，用猴肾细胞组蛋白F3，F2B和F2A1。没有与组蛋白F1共同电泳的病毒多肽。多肽VP4,5和6缺少色氨酸，并且仅由Vivo硫酸钠聚丙烯酰胺凝胶电泳测定的VP4含有半胱氨酸，其在体内用（3H）赖氨酸和（14C）色氨酸或（35s）胱氨酸（35s）胱氨酸。所有胶囊多肽Vp1,2和3含有色氨酸，而仅vp1和2含有半胱氨酸。此外，与用氨基酸的混合物标记的病毒相比，VP4,5和6富含精氨酸和赖氨酸。在感染之前标记的细胞中生长的病毒分析表明，VP4,5和6标记为大于大胶囊多肽VP1的五倍，这表明它们部分地衍生自预先存在的细胞组蛋白。基于这些数据和先前确定的分子量估计，我们得出结论，VP4,5和6分别是组蛋白F3，F2B和F2A1，但是不能排除SV40含有少量F2A2的可能性。

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