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RNA-directed DNA polymerase activity of reticuloendotheliosis virus: characterization of the endogenous and exogenous reactions.

机译:网状细胞病毒的RNA定向DNA聚合酶活性:内源性和外源反应的表征。

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Reticuloendotheliosis virus (REV) contains an endogenously instructed, RNA-directed DNA polymerase activity. Both the endogenous and exogenous DNA polymerase activities exhibited up to 10-fold greater activity at the optimum concentration of manganous ion (0.025 mM for exogenous; 0.25 mM for endogenous) than at any concentration of magnesium ion. Antiserum to the DNA polymerase of an REV group virus (spleen necrosis virus) inhibited both endogenous and exogenous DNA polymerase activity of REV, whereas antiserum to the Rous sarcoma virus (Rous-associated virus-0) [RSV(RAV-0)]DNA polymerase did not. The DNA product of the endogenous reaction is associated with the high-molecular-weight RNA of REV and anneals with REV RNA but not with RNA from Rous sarcoma virus.
机译:网状细胞病毒(Rev)含有内源性指导的RNA导向的DNA聚合酶活性。内源性和外源性DNA聚合酶的活性在锰离子的最佳浓度(0.025mm的外源的0.025mm的情况下,其均具有高达10倍的活性,而不是在任何浓度的镁离子中的浓度。转血病毒(脾脏坏死病毒)的DNA聚合酶(脾脏坏死病毒)的抗血清抑制了Rev的内源性和外源性DNA聚合酶活性,而ROS肉瘤病毒(Rous相关病毒-0)的抗血清[RSV(RAV-0)] DNA聚合酶没有。内源反应的DNA产物与REV和退火的高分子量RNA与REV RNA的退火相关有关,但不具有来自肉瘤病毒的RNA。

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