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Synthesis of Viral-Specific RNA in Cells Infected with the Parvovirus, Kilham Rat Virus

机译:用细胞膜病毒,Kilham大鼠病毒感染细胞中病毒特异性RNA的合成

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We have studied the viral-specific RNA synthesized after infection of a permissive cell line with Kilham rat virus (KRV). The RNA was shown to be virus specific by analysis of its nucleotide base ratios and by hybridization with KRV and cellular DNA. Viral RNA is synthesized as early as 2 h after infection. This viral RNA synthesis occurs before viral progeny DNA synthesis which is initiated at 7 to 8 h after infection. The predominant viral RNA synthesized before and after viral progeny DNA synthesis has a sedimentation coefficient of approximately 18S in dimethylsulfoxide-sucrose gradients and a calculated molecular weight of 6.5 × 105 to 7.5 × 105. KRV contains a molecule of single-stranded DNA with a molecular weight of approximately 1.6 × 106. If the viral-specific 18S RNA is a homogenous species, it would account for 40 to 50% of the viral genome. A small amount of 26S viral RNA with a molecular weight of 1.6 × 106 to 1.7 × 106 can also be detected. If this 26S RNA is a single viral-specific entity, it could represent a transcription of the entire KRV genome.
机译:我们研究了在允许千米大鼠病毒(KRV)感染允许细胞系后合成的病毒特异性RNA。通过分析其核苷酸基础比率和用KRV和细胞DNA杂交来显示RNA是特异性的病毒。感染后2小时,病毒RNA合成。该病毒RNA合成在病毒后代DNA合成前发生,感染后7至8小时在7至8小时开始。在病毒后代DNA合成之前和之后合成的主要病毒RNA在二甲基硫氧化物 - 蔗糖梯度中具有约18℃的沉降系数,并且计算的分子量为6.5×10 5 7.5×10 5 。 KRV含有单链DNA分子,其分子量为约1.6×10 6 。如果特异性特异性18 S RNA是均质物种,则会占病毒基因组的40%至50%。也可以检测少量的26/20S 病毒RNA,其分子量为1.6×10 6 至1.7×10 6-sup>。如果该26 S RNA是单一病毒特异性实体,它可以代表整个KRV基因组的转录。

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