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首页> 外文期刊>Journal of Virology >Polypeptide Composition of Urea- and Heat-Resistant Mutants of Poliovirus Types 1 and 2
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Polypeptide Composition of Urea- and Heat-Resistant Mutants of Poliovirus Types 1 and 2

机译：Poliovirus类型1和2的尿素和耐热突变体的多肽组合物

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Five urea-resistant and two heat-resistant mutants of poliovirus types 1 and 2 were isolated and their structural and nonstructural polypeptides compared to those of their wild-type, parental strains in an attempt to correlate mutant phenotypes with alterations in specific capsid polypeptides. Four of the seven mutants were found to contain polypeptides which differed in molecular weight from their respective parental viruses. However, resistance of virions to heat- or urea-inactivation could not be attributed to changes in particular capsid polypeptides because alterations were detected in all but one of the capsid components. For two of the urea-resistant mutants and one heat-resistant mutant, no differences were found in the molecular weights of the capsid and noncapsid polypeptides. These results, and the fact that at least 12 selective treatments were required to obtain stable mutants, indicate that: (i) such phenotypes probably can be expressed by mutations affecting one or more of the larger capsid polypeptides, and (ii) such phenotypes reflect multiple mutational steps.
机译：与它们的野生型治疗菌株相比，分离出五种尿素病毒类型1和2的耐热性突变体，并与其野生型的那些，试图将突变表型与特定衣壳多肽的改变相关。发现七个突变体中的四个含有从其各自的父母病毒中分子量不同的多肽。然而，病毒素对热或尿素灭活的抵抗力不能归因于特定衣壳多肽的变化，因为在所有胶囊组分中除了除其中之外的所有之外的改变。对于两种尿素突变体和一种耐热突变体，在衣壳和非携带多肽的分子量中没有发现差异。这些结果以及至少需要12种选择性处理来获得稳定突变体的事实表明：（i）此类表型可以通过影响一种或多种较大衣壳多肽的突变表达，并且（II）这种表型反射多个突变步骤。

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