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首页> 外文期刊>Journal of Virology >DNA-Dependent RNA Polymerase Activity Associated with Subviral Particles of Polyhedral Cytoplasmic Dexoyribovirus
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DNA-Dependent RNA Polymerase Activity Associated with Subviral Particles of Polyhedral Cytoplasmic Dexoyribovirus

机译：与多面体细胞质Dexoyribovirus的亚病毒颗粒相关的DNA依赖性RNA聚合酶活性

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摘要

Polyhedral cytoplasmic deoxyribovirus virions contain a DNA-dependent RNA polymerase which catalyzes the incorporation of ribonucleotides into an acid-precipitable product. Treatment of virions with sodium deoxycholate and dithiothreitol resulted in the formation of subviral particles which could be separated from virions by rate zonal centrifugation in sucrose gradients. Subviral particles were RNA polymerase-positive and more active per unit mass of protein than virions. In vitro enzyme activity associated with subviral particles required addition of ribonucleotides, Mg2+, and exogenous denatured DNA template. Optimal enzyme activity occurred over a broad pH (7.2 to 8.8) and Mg2+ concentration (2 to 10 μmol) range. The specific activity of the RNA polymerase was maximal at 37 C. Addition of DNase or actinomycin D to the reaction mixture reduced the incorporation of [3H]UMP into an acid-precipitable product. The product of the reaction was sensitive to degradation by RNase but not to DNase or Pronase. These data suggest that the enzyme copies DNA into RNA.
机译：多面体细胞质脱氧病毒病毒虫含有DNA依赖性RNA聚合酶，其催化核糖核苷酸掺入酸性劣蛋白产物中。用脱氧胆酸钠和二硫代噻唑治疗病毒粒子导致亚病毒颗粒的形成，其可以通过蔗糖梯度的速率区分离心分离成病毒粒子。亚病态颗粒是RNA聚合酶阳性和每单位质量蛋白质的阳性阳性和更活跃的蛋白质。与亚病毒颗粒相关的体外酶活性需要添加核糖核苷酸，Mg 2 + 和外源性变性DNA模板。在宽pH（7.2至8.8）和Mg 2 + / sop>浓度（2至10μmol）范围内发生最佳酶活性。 RNA聚合酶的比活性在37℃下最大。向反应混合物中加入DNA酶或放线霉素D将[ 3℃> H] UMP掺入酸性劣割的产物中。反应的产物对RNase降解但不敏感但不敏感，但不依赖于dNase或扑灭。这些数据表明酶将DNA复制到RNA中。

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