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首页> 外文期刊>Journal of Virology >Nucleotide Sequence Relationships of Avian RNA Tumor Viruses: Measurement of the Deletion in a Transformation-Defective Mutant of Rous Sarcoma Virus
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Nucleotide Sequence Relationships of Avian RNA Tumor Viruses: Measurement of the Deletion in a Transformation-Defective Mutant of Rous Sarcoma Virus

机译：禽RNA肿瘤病毒的核苷酸序列关系：Rous Sarcoma病毒转化缺陷突变体中缺失的测量

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Stocks of cloned helper-independent Rous sarcoma virus (RSV) spontaneously segregate transformation-defective (td) mutants that appear to have an RNA genome composed of smaller subunits than those of the patent virus. Differential hybridization and competitive hybridization techniques involving reactions between viral RNA and proviral sequences in host cell DNA (under conditions of initial DNA excess) were used to measure the extent of the deletion in a td mutant of Prague strain (Pr) of RSV (Pr RSV-C). Viral 60 to 70S RNA sequences labeled to 1 to 5 × 107 counts per min per μg with 125I were characterized with respect to their properties in hybridization reactions and used to reinforce data obtained with [3H]RNA of lower specific activity. By these techniques, about 13% ± 3% of the sequences Pr RSV-C that formed hybrids with DNA from virus-induced sarcomas appeared to be deleted from the genome of td Pr RSV-C. Studies comparing hybridization of RNA from Pr RSV-C and td Pr RSV-C with RSV-related sequences in normal cells, and competition experiments with RNA from the endogenous chicken oncornavirus Rous-associated virus type 0 (RAV-0) provided evidence that the majority, if not all, of the RNA sequences of Pr RSV-C deleted from its transformation-defective mutant are not represented in normal chicken DNA. Competition studies with a leukosis virus, RAV-7, indicated this virus also lacks a genome segment of about the same size as the deletion in the td mutant. Finally, the genome of all three “exogenous” viruses was found to lack a small segment (about 12%) of sequences present in the endogenous provirus of RAV-O.
机译：克隆辅助无关的Rous肉瘤病毒（RSV）的股票自发地隔离转化缺陷（ Td ）突变体，其似乎具有比专利病毒的亚基组成的RNA基因组。涉及宿主RNA与宿主细胞DNA（在初始DNA过量的条件下）在宿主RNA和荧光序列之间反应的差异杂交和竞争杂交技术用于测量布拉格菌株的突变体中缺失的程度（ RSV（PR RSV-C）的PR）。将病毒60至70至70 S RNA序列标记为1至5×10 7 / sup>每分钟/μg的每分钟，具有 12 5 i的表征，其特征在于它们杂交反应中的性质，用于加强用[ 3 h] RNA获得的数据较低的特异性活性。通过这些技术，约13％±3％的序列PR RSV-C与来自病毒诱导的肉瘤的DNA形成杂种的序列PR RSV-C似乎从 Td Pr RSV-C的基因组中删除。与普通细胞中RSV相关序列的RNA与rγs相关序列的RNA杂交的研究，以及来自内源鸡肉病毒的RNA竞争实验0（Rav -0）提供了从其转化缺陷突变体中删除的PR RSV-C的大多数，如果并非所有人的证据表明，则不代表在正常的鸡DNA中。竞争与白血病病毒，RAV-7的研究表明，这种病毒也缺乏大约与 Td 突变体中缺失相同大小的基因组片段。最后，发现所有三种“外源”病毒的基因组缺乏存在于RAV-O的内源性潜隐症中存在的小段（约12％）序列。

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