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Characteristics of PRD1, a Plasmid-Dependent Broad Host Range DNA Bacteriophage

机译:PRD1的特征,质粒依赖性宽宿主范围DNA噬菌体

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Several distinctive properties of PRD1, an icosahedral plasmid-dependent phage, are described. The drug-resistance plasmid-dependent host range of PRD1 extends beyond the P incompatibility group and includes gram-negative bacteria containing plasmids of incompatibility groups N and W. PRD1 phage will infect pseudomonads and Enterobacteriaceae containing either a P or W incompatibility group plasmid. PRD1 adsorbs to the cell wall of R+ bacteria and thus its infectivity indicates cell wall alterations by these drug-resistance plasmid groups. PRD1 nucleic acid is duplex DNA with an estimated molecular weight of 24 × 106. The appearance of PRD1 in electron micrographs is suggestive of lipid content in addition to its buoyant density of 1.348 in CsCl and its sensitivity to chloroform. The latent period of PRD1 varies with the R+ host bacterial strain used for growth of the phage.
机译:描述了PRD1,ICosaheDral依赖性噬菌体的几种独特性质。 PRD1的耐药质粒依赖性宿主范围延伸超过P不相容性基团,包括含有不相容性组N和W.PRD1噬菌体的含革质粒的革兰氏阴性细菌,将感染掺杂副组织和肠杆菌酸痛,含有p或W不相容的群体质粒。 PRD1吸附到R + 细胞的细胞壁,因此其感染性表示通过这些耐药质粒基团的细胞壁改变。 PRD1核酸是双链DNA,估计分子量为24×10 6 。除了CSCL中1.348的浮力密度外,PRD1在电子显微照片中的出现是脂质含量的提示,并且其对氯仿的敏感性。 PRD1的潜伏期随着用于噬菌体生长的R + 宿主细菌菌株而变化。

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