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首页> 外文期刊>Journal of Virology >Early Biochemical Events Occurring After Infection of Bacillus cereus 569-SP1 with Bacteriophage GSW
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Early Biochemical Events Occurring After Infection of Bacillus cereus 569-SP1 with Bacteriophage GSW

机译：用噬菌体GSW感染芽孢杆菌569-SP1后发生早期生化事件

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摘要

After infection of Bacillus cereus 569-SP1 with the 5-hydroxymethyluracil-containing phage GSW, new dTTPase, dUTPase, and dUMP-hydroxymethylase activities appear. No significant changes in activities of other pyrimidine ribonucleoside or 2′-deoxyribonucleoside triphosphate nucleotidohydrolases were detected. dUTP and dUMP inhibit the dTTPase activity, whereas dTTP failed to inhibit dUTPase activity. The Km value for the substrate dUTP is 10?4 M and for dTTP is 4.85 × 10?4 M. Thymidylate synthetase activity is inhibited only when cells are infected during the late lag or very early log phases of growth; when cells are infected with phage during mid-log, thymidylate synthetase activity is unaffected. The data support the suggestion that, although phage GSW may inhibit an otherwise expected increase in activity of thymidylate synthetase, it fails to affect the already existing activity. The data presented do not allow discrimination as to whether the phage specifies inhibition of de novo synthesis of thymidylate synthetase or the increase in activity of already existing but not fully expressed enzyme.
机译：用含5-羟甲基脲噬菌体GSF，新的DTTPAse，utPase和倾卸羟甲基酶活性感染芽孢杆菌腺癌 569-SP1。检测到其他嘧啶核糖核苷或2'-脱氧核苷的活动的显着变化检测到三磷酸三磷酸核苷酸酶。 DUTP和DAVE抑制DTTP酶活性，而DTTP未能抑制DUTPASE活动。基板DUTP的 k m 值为10 Δ4 m和dttp为4.85×10 β4 M.只有当细胞在晚期滞后或生长的非常早期的对数阶段感染细胞时，才抑制胸苷酸盐合成酶活性;当在中木质期间用噬菌体感染细胞时，胸苷酸盐合成酶活性不受影响。数据支持建议，尽管噬菌体GSF可能抑制胸苷合成酶的活性的另外预期的预期增加，但它不能影响已经存在的活性。所提出的数据不允许识别噬菌体是否指定抑制Novo合成胸苷合成酶或已经存在但不完全表达酶的活性的增加。

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