首页> 外文期刊>Journal of Virology >Production of B-tropic murine leukemia virus by somatic cell hybrids between mouse peritoneal macrophages and simian virus 40-transformed human cells.
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Production of B-tropic murine leukemia virus by somatic cell hybrids between mouse peritoneal macrophages and simian virus 40-transformed human cells.

机译:通过小鼠腹膜巨噬细胞和猿猴病毒40转化的人细胞的体细胞杂种生产B-热带鼠白血病病毒。

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Simian virus 40 (SV40)-transformed human cells (LN-SV) were fused with BALB/c peritoneal macrophages (BALB/c X LN-SV) and with C57BL peritoneal macrophages (C57BL X LN-SV) and hybrid clones, all of which had segregated human chromosomes and contained the entire complement of mouse chromosomes, were isolated. All 15 BALB/c X LN-SV hybrid clones were producing varying titers (10 to 10(6) plaque-forming units/ml) of B-tropic murine leukemia virus, whereas none of the nine C57BL X LN-SV hybrid clones was producing detectable ecotropic murine leukemia virus.
机译:Simian病毒40(SV40) - 转化的人体细胞(LN-SV)与BALB / C腹膜巨噬细胞(BALB / C X LN-SV)融合,并用C57BL腹膜巨噬细胞(C57BL X LN-SV)和杂交克隆已经分离了偏析人染色体并含有小鼠染色体的整个补体。所有15 Balb / C X LN-SV杂化克隆都生产不同滴度(10至10(6)个形成单位/ ml)B-Tropic鼠白血病病毒,而九个C57BL X LN-SV杂交克隆则不是生产可检测的生态鼠白血病病毒。

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