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首页> 外文期刊>Journal of Virology >Induction of Type C Virions from Normal Rat Kidney Cells by 2-Deoxy-d-Glucose
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Induction of Type C Virions from Normal Rat Kidney Cells by 2-Deoxy-d-Glucose

机译：用2-脱氧-D-葡萄糖从正常大鼠肾细胞诱导C型中的病毒粒子

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The sugar 2-deoxy-d-glucose (2-DG) induced the release of type C virions from an established line of normal rat kidney (NRK) cells. Within 20 h after the addition of 5 mg of 2-DG per ml to exponentially growing NRK cultures, more than 80% of the cells expressed the mammalian type C virus interspecies-specific antigen (p30) as determined by indirect cytoplasmic immunofluorescence. Maximal virion release occurred 1 to 2 days after 2-DG was added for 24 h to the growth medium, although a low level of virion production was detected as early as 2.5 h after 2-DG treatment. Studies with inhibitors of RNA synthesis indicated a requirement for de novo RNA synthesis after the addition of 2-DG. Sensitivity of NRK cells to type C virion induction was limited to a relatively short period of in vitro growth and preceded spontaneous virion release by 8 to 10 subculture generations. A model is presented for the sequential derepression of latent type C virus information in serially propagated NRK cells.
机译：糖2-脱氧-d-葡萄糖（2-DG）诱导C型病毒型从正常大鼠肾（NRK）细胞的成熟线释放。在20小时内加入5mg每mL的2-DG为指数增长的NRK培养物后，超过80％的细胞表达了通过间接细胞质免疫荧光确定的特异性抗原（P30）的哺乳动物型病毒。在将2-DG加入24小时后，最大的病毒粒子释放发生1至2天，但在2-DG处理后早在2.5小时检测到低水平的病毒粒子生产。 RNA合成抑制剂的研究表明添加2-DG后的Novo RNA合成的要求。 NRK细胞对C型病毒酮诱导的敏感性仅限于体外生长的相对短的时间，并且前面的自发性病毒素释放到8至10代培养代。提出了一种模型，用于串联传播的NRK细胞中潜伏的C病毒信息的顺序DERE倾向。

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