首页> 外文期刊>Journal of Virology >In Vitro Transcription of 70S RNA by the RNA-Directed DNA Polymerase of Rous Sarcoma Virus: Lack of Influence of RNase H
【24h】

In Vitro Transcription of 70S RNA by the RNA-Directed DNA Polymerase of Rous Sarcoma Virus: Lack of Influence of RNase H

机译:由RNA的RNA定向的DNA聚合酶对70s RNA的体外转录:RNase H的影响缺乏影响

获取原文
           

摘要

The influence of Rous sarcoma virus (RSV)-associated RNase H on the in vitro synthesis of DNA by the RSV RNA-directed DNA polymerase was determined under conditions whereby RNase H activity was selectively inhibited with NaF. Not only were we unable to detect any effect on the size, structure, or genetic complexity of the DNA product synthesized in the absence of RNase H activity, but the displacement of DNA from the 70S RNA:DNA hybrid structures was also unaffected. The suitability of 70S RNA:DNA hybrid structures synthesized in vitro to serve as a substrate for RNase H is discussed.
机译:在RSV RNA定向的DNA聚合酶中,在NAF选择性地抑制RNase H活性的条件下,测定ROS肉瘤病毒(RSV)的对DNA的体外合成的影响。不仅我们无法检测到在不存在RNase H活性的DNA产品的大小,结构或遗传复杂性的任何影响,但DNA从70 S RNA:DNA的移位杂化结构也不受影响。讨论了70 S RNA:在体外合成的DNA杂化结构用作RNase H的底物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号