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首页> 外文期刊>Journal of Virology >Bacteriophage and bacteriophage-like structures carried by Bacillus medusa and their effect on sporulation.
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Bacteriophage and bacteriophage-like structures carried by Bacillus medusa and their effect on sporulation.

机译：芽孢杆菌培养的噬菌体和噬菌体样结构及其对孢子的影响。

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Bacillus medusa was found to carry three phages or phagelike structures named phi med-1, phi med-2, and phi med-3. phi med-1 is a minute, 25-nm-diameter particle without a tail. It was extracted from the sporulation lysate of a phi med-2-minus strain of B. medusa and purified by differential centrifugation. The nucleic acid from this structure was shown to be orcinol positive, alkali sensitive, RNase sensitive, and DNase resistant. An RNase-resistant core of nucleic acid was not found, indicating that it was single-stranded RNA. A host strain has not yet been found for phi med-1. Phage phi med-3 was induced with mitomycin C or UV light and consisted of empty heads of 57 nm in diameter, whereas phi med-2 induced with mitomycin was a phage of 60-nm head diameter and 220-nm tail length. The sporulation sequence proceeded faster in those mutants lacking phi med-2, and when the phage was reintroduced to B. medusa the extended wild-type sporulation sequence was observed. B. thuringiensis var. schwetzova was sensitive to phi med-2 and yielded small turbid plaques. B. medusa produced small numbers of phi med-2 during growth. The other phage may be produced at the same time but were not detected. Phi med-1 was found in sporulating cells by electron microscopy techniques. Its release from these was demonstrated by both electron microscopy techniques and a radioactive assay. It appears to participate in the formation of a surface layer on the parasporal inclusion of B. medusa.
机译：发现芽孢杆菌培养培养三个噬菌体或氏丸结构，名为phi med-1，phi med-2和phi med-3。 PHI MED-1是一分钟，直径为25纳米的粒子，没有尾部。从B. medusa的Phi Med-2-minus菌株的孢子裂解物中提取，并通过差动离心纯化。来自该结构的核酸显示为orcinol阳性，碱敏感，RNase敏感和DNase抗性。未发现核酸的耐rNase抵抗核心，表明它是单链RNA。尚未发现宿主应变为PHI Med-1。噬菌体PHI MED-3用丝霉素C或UV光诱导，并由直径为57nm的空头组成，而用丝霉素诱导的PHI MED-2是60-nm的噬菌体，220nm尾长。散发序列在缺乏PHI MED-2的突变体中进行更快，并且当噬菌体重新掺杂到B. MEDUSA时，观察到延长的野生型孢子序列。 B. thuringiensis var。 Schwetzova对Phi Med-2敏感，并产生了小的混浊斑块。 B. Medusa在生长期间产生了少量的PHI Med-2。可以同时生产其他噬菌体但未检测到。通过电子显微镜技术在孢子用细胞中发现PHI med-1。通过电子显微镜技术和放射性测定来证明它们的释放。它似乎参与了B. memusa的诱惑夹杂物上的表面层的形成。

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