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Viral gene expression in murine sarcoma virus(murine leukemia virus)-infected cells.

机译:在鼠肉瘤病毒(鼠白血病病毒)的病毒基因表达 - 摄入细胞。

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NIH 3T3 cells infected with Moloney murine sarcoma virus (murine leukemia virus) produce virions which contain about 99% murine sarcoma virus RNA and 1% murine leukemia virus RNA. This report describes experiments which measured intracellular concentrations of proviral DNA and RNA transcripts for each of the viruses. We found that three to four copies of proviral DNA from each virus were integrated into the cellular DNA. Measurements of RNA specific for each of the genomes by hybridization to specific cDNA reagents revealed a 10- to 15-fold difference in concentration in both nuclear and polysomal RNA fractions, with murine sarcoma virus RNA predominating in both cases. Unless there are major differences in stability between the two viral RNAs, our results suggest that transcriptional control is responsible for much of the difference in final levels of virus synthesis.
机译:NIH 3T3细胞感染Moloney Murine Sarcoma病毒(鼠白血病病毒)产生含有约99%鼠肉瘤病毒RNA和1%鼠白血病病毒RNA的病毒粒子。本报告描述了对每个病毒测量的荧光DNA和RNA转录物的细胞内浓度的实验。我们发现,将来自每个病毒的三到四个普通DNA拷贝整合到细胞DNA中。通过杂交对特异性cDNA试剂的每个基因组的RNA测量显示核和多血症RNA级分的浓度10-15倍,鼠肉瘤病毒RNA在两种情况下占主导地位。除非两种病毒RNA之间的稳定性有重大差异,否则我们的结果表明转录控制负责病毒合成的最终水平的大部分差异。

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  • 来源
    《Journal of Virology》 |1978年第3期|共8页
  • 作者

    D Dina; E E Penhoet;

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  • 收录信息 美国《科学引文索引》(SCI);
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