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首页> 外文期刊>Journal of Virology >Bacteriophage P22-mediated specialized transduction in Salmonella typhimurium: identification of different types of specialized transducing particles.
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Bacteriophage P22-mediated specialized transduction in Salmonella typhimurium: identification of different types of specialized transducing particles.

机译：噬菌体P22介导的沙门氏菌血吸虫的专业转导：鉴定不同类型的专用换粒子。

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摘要

The temperate bacteriophage P22 mediates both generalized and specialized transduction in Salmonella typhimurium. Specialized transduction by phage P22 is different from, and less restricted than, the well characterized specialized transduction by phage lambda, due to differences in the phage DNA packaging mechanism. Phage lysates produced by induction of lysogenic strains contain very high frequencies of supQ newD- and proA,B-specialized transducing particles (10(-2)/PFU and 10(-3)/PFU, respectively), most of which are produced by independent aberrant excision events of various types. In a model, 12 different modes of transduction mechanisms were characterized by: (i) the structure of the specialized transducing genomes after injection into a new host cell, i.e., linear or circular, and (ii) the requirements for the transduction process, i.e., host recombination functions, phage integration functions, or presence of a prophage. By using different recipient strains and phage helper strains, it was possible to show that most specialized transducing particles (ca. 99%) contain linear genomes that cannot circularize upon injection into a new host cell and that require the presence of an integrated prophage as a site for a recombinational event to give rise to a transductant. Only 0.1% of all specialized transducing particles were shown to transduce by integration, suggesting that transducing genomes containing terminally redundant ends represent only a minor fraction of all transducing particles that are produced. However, it should be pointed out that the frequency (approximately 10(-5)/PFU) of these specialized transducing genomes that can circularize upon injection into a new host cell is as high as or even higher than the frequency of specialized transducing particles of phage lambda. The remaining approximately 1% of all specialized transducing particles can transduce by any one of the other mechanisms described.
机译：温带噬菌体P22介导在沙门氏菌血硫尿中的广义和专门的转导。由于噬菌体DNA包装机制的差异，噬菌体P22的专门转导与噬菌体λ的良好特异性转导的不同。通过诱导溶菌菌株产生的噬菌体裂解物含有非常高的SupQ Newd-and Proa，B专用转换颗粒（10（-2）/ pfu和10（-3）/ pfu），其中大部分由各种类型的独立异常切除事件。在模型中，通过：（i）注射到新的宿主细胞中的专用转换基因组的结构，即线性或圆形，以及（ii）转导工艺的要求，即：（i）的结构的12种不同的转导机构的结构，主机重组函数，噬菌体集成功能，或者存在的存在。通过使用不同的受体菌株和噬菌体辅助菌株，可以表明，最专业的转换颗粒（CA.99％）含有线性基因组，其不能在注射到新的宿主细胞时圆形化，并且需要存在综合的网点作为一个用于重组事件的网站，以产生转置剂。仅通过整合显示只有0.1％的所有专门的换颗粒进行转导，表明含有终端冗余末端的转换基因组仅表示产生的所有换颗粒的少量部分。然而，应该指出的是，这些专用转换基因组的频率（大约10（-5）/ pfu）可以在注射到新的宿主细胞时圆形化的是高于或甚至高于专用转换颗粒的频率噬菌体λ。剩余的约1％的所有专门的换颗粒都可以通过所描述的其他机制中的任何一种来转导。

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    《Journal of Virology》 |1978年第3期|共16页
  • 作者

    D Y Kwoh; J Kemper;

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  • 收录信息 美国《科学引文索引》(SCI);
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