Peptide mapping characterization of viral proteins generated in a cell-free coupled system for the transcription and translation of influenza virus mRNA.

J Content; L De Wit; M A Horisberger

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Peptide mapping characterization of viral proteins generated in a cell-free coupled system for the transcription and translation of influenza virus mRNA.

机译：在无细胞偶联系统中产生的病毒蛋白的肽测绘表征，用于流感病毒mRNA的转录和翻译。

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In a coupled cell-free system for the transcription and translation of the influenza mRNA's, containing detergent-disrupted purified NWS influenza virion and a micrococcal nuclease-preincubated rabbit reticulocyte lysate, five unglycosylated viral proteins (NS1, M, NP, P1, and P3) were easily produced and isolated. Their identification was based on the electrophoretic separation of peptide fragments resulting from their partial digestion with proteases of restricted specificity (D.W. Cleveland, S. G. Fisher, N. W. Kirschner, and U. K. Laemmli, J. Biol. Chem. 252:1102-1106, 1977).

机译：在耦合的无细胞系统中，用于流感型MRNA的转录和翻译，含有洗涤剂破坏的纯化的NWS流感病毒虫和微球菌核酸酶 - 预孵育的兔网裂解物，五种不凝胶化病毒蛋白（NS1，M，NP，P1和P3 ）容易产生和分离。它们的鉴定基于肽片段的肽片段与受限制特异性的蛋白酶产生的肽片段的电泳分离（D.W.Cleveland，S. G.Fisher，N.W.Kirschner，以及美国，J.Biol。化学。252：1102-1106，1977）。

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《Journal of Virology》 |1978年第3期|共5页
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J Content; L De Wit; M A Horisberger;
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收录信息美国《科学引文索引》(SCI);
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1. Peptide mapping characterization of viral proteins generated in a cell-free coupled system for the transcription and translation of influenza virus mRNA. [J] . J Content, L De Wit, M A Horisberger Journal of Virology . 1978,第3期

机译：在无细胞偶联系统中产生的病毒蛋白的肽测绘表征，用于流感病毒mRNA的转录和翻译。
2. Peptide mapping characterization of viral proteins generated in a cell-free coupled system for the transcription and translation of influenza virus mRNA. [J] . J Content, L De Wit, M A Horisberger Journal of Virology . 1978,第3期

机译：在无细胞偶联系统中产生的病毒蛋白的肽测绘表征，用于流感病毒mRNA的转录和翻译。
3. Cell-free translation of influenza virus mRNA. [J] . J Content Journal of Virology . 1976,第2期

机译：流感病毒mRNA的无细胞翻译。
4. Mammalian In Vitro Translation (Cell-Free) Systems for Rapid and High-Yield Expression of Functional Proteins Including Antibodies [C] . Penny Jensen, Krishna Vattem, Justin Saul, PEGS . 2015

机译：哺乳动物体外翻译（无细胞）系统，用于快速和高产功能蛋白质表达，包括抗体
5. The Roles of Viral and Host Proteins in Influenza A Virus Assembly and Budding in Infected Cells [D] . Bedi, Sukhmani Kaur. 2019

机译：病毒和宿主蛋白在流感病毒组装和萌芽中的作用
6. Peptide mapping characterization of viral proteins generated in a cell-free coupled system for the transcription and translation of influenza virus mRNA. [O] . J Content, L De Wit, M A Horisberger 1978

机译：在无细胞偶联系统中产生的病毒蛋白的肽图表征用于流感病毒mRNA的转录和翻译。
7. Peptide mapping characterization of viral proteins generated in a cell-free coupled system for the transcription and translation of influenza virus mRNA [O] . J Content, L De Wit, M A Horisberger 1978

机译：在无细胞偶联系统中产生的病毒蛋白的肽测绘表征，用于流感病毒mRNA的转录和翻译

Peptide mapping characterization of viral proteins generated in a cell-free coupled system for the transcription and translation of influenza virus mRNA.

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