• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Virology >Characterization of La Crosse virus small-genome transcripts.
【24h】

Characterization of La Crosse virus small-genome transcripts.

机译：La Crosse病毒小基因组转录物的表征。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

With restriction fragments from DNA clones of the La Crosse virus S genome segment, the 3' end of the S mRNA was located by S1 nuclease mapping near a polyuridine tract, approximately 100 nucleotides, from the end of the S genome. Genome replication in La Crosse virus-infected cells was abolished by the drug cycloheximide, similar to other negative-strand RNA viruses. However, the synthesis of S mRNA could not be detected in cells pretreated with cycloheximide, suggesting that ongoing protein synthesis is required for La Crosse virus genome transcription and replication. Primer extension experiments in the presence of chain-terminating nucleoside triphosphates demonstrated that the 5' end of the La Crosse virus S mRNA begins 10 to 14 nucleotides before the 3' end of the S genome segment, suggesting that the La Crosse virus S mRNA is initiated on a host primer. A hypothesis consistent with these unexpected findings is presented.
机译：利用来自La Crosse病毒S基因组区段的DNA克隆的限制性片段，S mRNA的3'末端通过S1核酸酶映射，从S基因组的末端靠近聚嘌呤道，大约100个核苷酸。通过药物环己酰亚胺废除了La Crosse病毒感染细胞的基因组复制，类似于其他阴茎RNA病毒。然而，在用环己酰亚胺预处理的细胞中不能检测到S mRNA的合成，表明La Crosse病毒基因组转录和复制需要正在进行的蛋白质合成。在链链终止核苷三磷酸盐存在下的引物延伸实验证明了La Crosse病毒S mRNA的5'末端在S基因组片段的3'末端之前开始10至14个核苷酸，表明La Crosse病毒S mRNA是在主题引物启动。提出了与这些意外发现一致的假设。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号