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首页> 外文期刊>Journal of Virology >Characterization of the 92,000-dalton glycoprotein induced by herpes simplex virus type 2.
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Characterization of the 92,000-dalton glycoprotein induced by herpes simplex virus type 2.

机译：单纯疱疹病毒2型患有92,000-Dalton糖蛋白的表征2。

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摘要

Evidence is presented showing that the 92,000-dalton glycoprotein (g92K) induced by herpes simplex virus (HSV) type 2 has properties distinct from those assigned to any other HSV glycoprotein. First, the carbohydrate composition and extent of sulfation differ from those of glycoproteins D and E. Second, two clonally unrelated monoclonal antibodies, AP1 and LP5, shown in this paper to specifically immunoprecipitate g92K, do not react with any of the known processed forms of glycoproteins B, C, D, and E. Third, by using HSV type 1/HSV type 2 intertypic recombinants and a simple radioimmunoassay, the target antigen of the two monoclonal antibodies was shown to map in the same region as g92K (0.846 to 0.924). Fourth, the intertypic recombinant R12-3 was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of infected cells to induce the HSV type 2 g92K and HSV type 1 gD and GE, whereas R12-1, which did not induce g92K, induced HSV-2 gE and an altered gD, providing genetic evidence that g92K is encoded, at least in part, by a different region of the genome from that encoding gD and gE.
机译：提出了证据表明，由疱疹病毒（HSV）2型蛋白诱导的92,000-Dalton糖蛋白（G92K）具有不同于分配给任何其他HSV糖蛋白的特性。首先，碳水化合物组成和硫化的程度与糖蛋白D和E.二，本文中所示的两个克隆不相关的单克隆抗体，AP1和LP5的两个克隆不相关的单克隆抗体，AP1和LP5，以特异性免疫沉淀G92K，不与任何已知的加工形式反应糖蛋白B，C，D和E.第三，通过使用HSV型1 / HSV型Intertypic重组剂和简单的放射免疫测定，两种单克隆抗体的靶抗原显示在与G92K相同的区域中映射（0.846至0.924 ）。第四，Intertypic重组R12-3由十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳的感染细胞显示，诱导HSV型2 G92K和HSV型1 GD和GE，而R12-1没有诱导G92​​K，诱导HSV- 2 GE和改变的GD，提供G92K至少部分地由基因组的不同区域从编码GD和GE进行编码的遗传证据。

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