• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Virology >A post-alpha gene function turns off the capacity of a host protein to bind DNA in cells infected with herpes simplex virus 1.
【24h】

A post-alpha gene function turns off the capacity of a host protein to bind DNA in cells infected with herpes simplex virus 1.

机译：后α基因功能关闭宿主蛋白的容量，以在感染疱疹病毒1感染的细胞中结合DNA。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

HEp-2 cell proteins electrophoretically separated in denaturing polyacrylamide gels and electrically transferred to nitrocellulose sheets contain a polypeptide which efficiently binds linear native DNA end labeled with 32P but not denatured DNA. The polypeptide has an apparent molecular weight of ca. 130,000. The activity of the protein was stable, and no appreciable turnover was observed after exposure of uninfected cells to inhibitory concentrations of cycloheximide for intervals of up to 24 h. However, the activity was absent from lysates of cells harvested 6 h or later postinfection with wild-type viruses. To identify the viral function involved in the loss of DNA-binding activity, we tested the lysates of cells infected with several mutants. Thus, the DNA-binding activity was unaffected in cells infected with a temperature-sensitive mutant (herpes simplex virus 1 tsLB2) in the alpha 4 gene and was maintained at a nonpermissive temperature (39 degrees C). Experiments involving (i) temperature shift-down of cells infected with tsLB2 in the presence of cycloheximide, (ii) withdrawal of cycloheximide in the presence and absence of actinomycin D from cells infected with wild-type virus, (iii) infection of cells at 33 and 39 degrees C with herpes simplex virus 1 tsHA1 carrying a temperature-sensitive lesion in the beta 8 gene, and (iv) infection of cells in the presence of inhibitory concentrations of phosphonoacetate led to the conclusion that the viral functions responsible for the loss of DNA-binding capacity were specified by either beta or gamma genes not dependent on viral DNA synthesis for their expression.
机译：在变性聚丙烯酰胺凝胶中电泳分离的HEP-2细胞蛋白质和电转移到硝酸纤维素片材中含有多肽，该多肽有效结合用32P标记的线性天然DNA末端，但不变性DNA。多肽具有CA的表观分子量。 130,000。蛋白质的活性稳定，并且在暴露于未感染的细胞后没有观察到可明显的周转，以抑制环己酰亚胺的浓度，以达到24小时的间隔。然而，通过用野生型病毒收获的细胞的裂解物不存在该活性。为了鉴定DNA结合活性丧失的病毒功能，我们测试了用几个突变体感染的细胞的裂解物。因此，DNA结合活性在α4基因中用温度敏感突变体（疱疹病毒1sLB2）感染的细胞中不受影响，并在非智能温度（39℃）处保持。涉及（i）在环己酰亚胺的存在下感染TSLB2感染的细胞的温度移位的实验，（ii）在感染野生型病毒感染的细胞存在和不存在的环己酰胺中脱离环己酰亚胺，（III）细胞感染33和39摄氏度用疱疹病毒1 TSHA1在β8基因中携带温度敏感病变，（IV）细胞的抑制浓度的膦酸浓度存在的结论导致了损失负责的病毒功能通过β或γ基因指定DNA结合能力，不依赖于其表达的病毒DNA合成。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号