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首页> 外文期刊>Journal of Virology >Interferon-mediated, double-stranded RNA-dependent protein kinase is inhibited in extracts from vaccinia virus-infected cells.
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Interferon-mediated, double-stranded RNA-dependent protein kinase is inhibited in extracts from vaccinia virus-infected cells.

机译：干扰素介导的双链RNA依赖性蛋白激酶被疫苗病毒感染细胞的提取物中抑制。

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The interferon-inducible, double-stranded RNA (dsRNA)-dependent protein kinase which phosphorylates an endogenous HeLa 69 kilodalton polypeptide or exogenous initiation factor eIF2 was inhibited during vaccinia virus infection. High interferon doses (20,000 reference units per ml) did not prevent this inhibition. The inhibition required protein synthesis but not viral DNA synthesis during infection, suggesting that an early vaccinia virus gene function was responsible. An active dsRNA-dependent protein kinase could be recovered from an inactive extract by purification on polyinosinate X polycytidylate-cellulose. An inhibitor of the protein kinase, therefore, must be present in the inactive extract. Similar results have been obtained with mouse L929 cells. At early time points of infection, the protein kinase in cell extracts required exogenous dsRNA for activity. This argues against endogenous viral dsRNA and activation of the kinase in the intact cell. At late time points of infection (when vaccinia virus dsRNA was almost certainly formed), the inhibitor of the kinase is present. Accordingly, it seems unlikely that the kinase played any role in the interferon-mediated inhibition of virus growth observed in these cells under these particular conditions.
机译：在疫苗病毒感染期间抑制了磷酸化内源HeLa 69千杆菌多肽或外源引发因子EIF2的干扰素诱导的双链RNA（DSRNA） - 依赖性蛋白激酶。高干扰素剂量（每mL的20,000个参考单位）没有阻止这种抑制。抑制所需的蛋白质合成但在感染过程中不是病毒性DNA合成，表明早期的疫苗病毒基因功能是负责任的。通过纯化在多咸氨酸X多环晶酯 - 纤维素上可以从非活性提取物中回收活性DSRNA依赖性蛋白激酶。因此，蛋白激酶的抑制剂必须存在于非活性提取物中。用小鼠L929细胞获得了类似的结果。在早期感染点，细胞提取物中的蛋白质激酶需要外源性DsRNA进行活性。这涉及内源性病毒DSRNA和完整细胞中激酶的激活。在迟到的感染点（当疫苗病毒DsRNA几乎肯定形成时），存在激酶的抑制剂。因此，Kinase似乎不太可能在这些特定条件下在这些细胞中观察到的病毒生长的病毒生长的任何作用。

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    《Journal of Virology》 |1984年第1期|共8页
  • 作者

    A P Rice; I M Kerr;

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  • 收录信息 美国《科学引文索引》(SCI);
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