Synthesis and properties of poly 5-methyltnionridylic acid

Yau-Kwan Ho

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Synthesis and properties of poly 5-methyltnionridylic acid

机译：聚5-甲基二硫代锡的合成与性质

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In an effort to search for good methods for the enzymatic synthesis of polynucleotIde analogs with antitemplate activity, 5-methylthlouridine-5′-diphosphate (ms5uDP) has been synthesized and Investigated as a substrate for polynucleotIde phosphorylase. While ms5uDP was polymerized at a very low rate to give a 6% yield of polynucleotides by the polynucleotide phosphorylase of Micrococcus luteus, it was utilized more efficiently by the corresponding enzyme of Escherichia coli resulting in a 15% yield of poly(5-methylthlo-uridylic) acid. Results of the co-polymerization of ms5uDP and UDP revealed that the ratio of 5-methyithiouridylate to uridylate residues in the polynuc-leotide product was lower than the ratio of ms5UDP to UDP In the substrate mixture. The 5-methylthio group conferred only minute changes on the conformation of the modified polyuridylic acid, and the complexes formed between poly-(5-methylthiourldylIc) acid and poly(adenylIc) acid possessed slightly higher Tm values than did the unmodified counterparts. Poly(5-methylthlouridylic) acid was a potent Inhibitor of calf thymus ONA polymerase α.

机译：在努力寻找酶促合成具有抗原膜蛋白活性的多核苷酸类似物的良好方法，已经合成了5-甲基酚-5'-二磷酸（MS 5℃> UDP），并作为多核苷酸磷酸化酶进行研究。虽然MS ^{5 UDP以非常低的速率聚合，但通过微球菌的多核苷酸磷酸化酶得到6％的多核苷酸产率，通过大肠杆菌的相应酶更有效地使用它15聚（5-甲基 - 尿嘧啶）酸的产率％。 MS 5 UDP和UDP的共聚结果显示，PolyNuc-Leotide产物中的5-甲基二硫代氨酸酯与甲酰酯残留物的比例低于MS ^{5 UDP在基材混合物中的UDP。该5-甲基硫基组仅赋予改性聚脲酰基的构象的微小变化，并且在聚 - （5-甲基硫基）酸和聚（腺苷）酸之间形成的复合物具有比未修饰的对应物略高的Tm值。聚（5-甲基律膦酸）酸是CALF胸腺ONA聚合酶α的有效抑制剂。}}

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《Nucleic acids research》 |1984年第19期|共16页
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Yau-Kwan Ho;
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1. Synthesis and properties of poly 5-methyltnionridylic acid [J] . Yau-Kwan Ho Nucleic acids research . 1984,第19期

机译：聚5-甲基二硫代锡的合成与性质
2. Synthesis and properties of poly 5-methyltnionridylic acid [J] . Yau-Kwan Ho Nucleic acids research . 1984,第19期

机译：聚5-甲基二硫代锡的合成与性质
3. Poly(2-acrylamidoglycolic acid-co-2-acrylamide-2-methyl-1-propane sulfonic acid) and poly(2-acrylamidoglycolic acid-co-4-styrene sodium sulfonate): synthesis, characterization, and properties for use in the removal of Cd(II), Hg(II), Zn(II), and Pb(II) [J] . Morales Daniela V., Rivas Bernabe L. Polymer bulletin . 2015,第2期

机译：聚（2-丙烯酰胺基乙醇酸-co-2-丙烯酰胺-2-甲基-1-丙烷磺酸）和聚（2-丙烯酰氨基乙醇酸-co-4-苯乙烯磺酸钠）：合成，表征和性能去除Cd（II），Hg（II），Zn（II）和Pb（II）
4. Synthesis and Properties of Biodegradable Liquid Crystalline Poly(ester-urethane)s Based on Poly(L-lactic acid) [C] . Yatong Guo, Zhu Zheng, Zhenqi Hou, International Conference on Advances in Materials and Manufacturing . 2013

机译：基于聚（L-乳酸）的可生物降解液晶聚（酯 - 氨基甲酸酯）S的合成与性能
5. BIOCHEMICAL STUDY OF POLY-ALPHA-L-GLUTAMIC ACID. PART I: THE EFFECT OF CONFORMATION ON THE CHEMICAL, PEPSIN OR PRONASE HYDROLYSIS OF POLY-ALPHA-L-GLUTAMIC ACID. PART II: BIOLOGICAL PROPERTIES OF POLY-ALPHA-L- GLUTAMIC ACID AND ITS DERIVATIVES. PART III: A NEW CALCIUM REQUIREMENT FOR THE HYDROLYSIS OF POLY-ALPHA-L-GLUTAMIC ACID BY THE CULTURE FILTRATESFROM BACILLUS LICHINOFORMIS. [D] . WAGLE, SUDHAKAR SHANTARAM. 1965

机译：聚α-L-谷氨酸的生物化学研究。第一部分：构象对聚-α-L-谷氨酸酸的化学，胃蛋白酶或蛋白酶水解的影响。第二部分：聚α-L-谷氨酸及其衍生物的生物学特性。第三部分：新的钙要求，用于培养滤液中的嗜酸芽孢杆菌（BACILLUS LICHINOFORMIS）水解聚-α-L-谷氨酸。
6. Polynucleotides. L. Synthesis and properties of poly (2-chloro-2-deoxyadenylic acid) and poly (2-bromo-2-deoxyadenylic acid). [O] . M Ikehara, T Fukui, N Kakiuchi 1977

机译：多核苷酸。 L.聚（2-氯-2-脱氧腺苷酸）和聚（2-溴-2-脱氧腺苷酸）的合成和性质。
7. Nucleotides. XX. Synthesis and properties of poly-2-alkyladenylic acids. III. Interactions with poly(X). [O] . MASAO HATTORI, WOLFGANG PFLEIDERER 1983

机译：核苷酸。 xx。聚2-烷基壬基酸的合成与性质。 III。与Poly（x）的相互作用。

Synthesis and properties of poly 5-methyltnionridylic acid

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