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首页> 外文期刊>The biochemical journal >A 17 β-hydroxysteroid dehydrogenase of female rabbit liver cytosol. Purification and characterization of multiple forms of the enzyme
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A 17 β-hydroxysteroid dehydrogenase of female rabbit liver cytosol. Purification and characterization of multiple forms of the enzyme

机译：雌性兔肝细胞溶胶的17β-羟类脱氢酶。酶多种形式的纯化和表征

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pMultiple forms of the soluble 17 beta-hydroxysteroid dehydrogenase of female rabbit liver were identified. NAD-dependent and NADP-dependent enzyme activities were separated by affinity chromatography on agarose-immobilized Procion Red HE3B, and three forms of the NADP-dependent enzyme activity were purified by chromatofocusing. These three enzyme forms are charge isomers and have no quaternary structure. The enzymes catalysed the C-17 oxidoreduction of oestrogens and androgens; with all enzyme forms the activity towards androgens was higher than that toward oestrogens. The enzymes also exhibited 3 alpha-hydroxysteroid dehydrogenase activity towards androgens of the 5 beta-androstane series. Comparison of the relative activities of the enzymes towards a number of oestrogen and androgen substrates revealed differences among the enzyme forms for both the oxidative and the reductive reactions. In particular, one enzyme form had a significantly lower Km for the 3 alpha-hydroxysteroid substrate and a higher 3 alpha-/17 beta-hydroxysteroid dehydrogenase activity ratio than the other two enzyme forms./p
机译：>鉴定了多种雌性兔肝脏的可溶性17β-羟类脱氢酶。通过琼脂糖 - 固定的涂布术红HE3B的亲和层析分离NAD依赖性和NADP依赖性酶活性，并通过染色体焦，纯化三种形式的NADP依赖性酶活性。这三种酶形式是电荷异构体，没有季结构。酶催化了雌激素和雄激素的C-17氧化氧化;所有酶都构成雄激素的活性高于OETTROGENS的活性。酶还表现出3个α-羟类脱氢酶活性朝向5β-甲醇甾烷系列的雌激素。将酶对许多雌激素和雄激素基质的相对活性的比较显示了氧化和还原反应的酶形式之间的差异。特别地，一种酶形式对于3个α-羟类甾醇基底具有显着低的km，以及更高的3α-/ 17β-羟类脱氢酶活性比比其他两种酶形式更低。

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