Methyl group analysis of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus.

D L Nuss; E Paoletti

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Methyl group analysis of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus.

机译：纯化痘苗病毒体外合成病毒型高分子重量RNA的甲基群分析。

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The methylation pattern of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus has been determined. Analysis of purified high-molecular-weight RNA synthesized with S-[methyl-3H]-adenosylmethionine and alpha[32P]UTP as precursors gave the following results. (i) Eessentially all molecules contained blocked and methylated structures of the type m7G(5')ppp(5')Gm and m7G(5')ppp(5')Am. (ii) There was no detectable methylation at internal sites. (iii) Under several different conditions of synthesis, the ratio of molecules containing m7G(5')ppp(5')Gm to those containing m7G(5')ppp(5')Am was imilar for both the virion-associated high-molecular-weight RNA and the virion-released 8-12S mRNA.

机译：确定了通过纯化的痘苗病毒合成体外的病毒型相关高分子重量RNA的甲基化模式。用S-α-烷基甲基硫氨氨酸和α[32P] UTP合成的纯化的高分子量RNA分析作为前体，得到以下结果。（i）偏离所有分子含有M7G（5'）PPP（5'）GM和M7G（5'）PPP（5'）am的封闭和甲基化结构。（ii）内部部位上没有可检测的甲基化。（iii）在几种不同的合成条件下，含有M7g（5'）ppp（5'）gm的分子与含有m7g（5'）ppp（5'）的比率对于病毒士相关的高 - 含有m7g（5'）ppp（5'）的比例是Imilar的。分子量RNA和病毒虫释放的8-12s mRNA。

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《Journal of Virology》 |1977年第1期|共7页
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D L Nuss; E Paoletti;
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收录信息美国《科学引文索引》(SCI);
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1. Methyl group analysis of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus. [J] . D L Nuss, E Paoletti Journal of Virology . 1977,第1期

机译：纯化痘苗病毒体外合成病毒型高分子重量RNA的甲基群分析。
2. Methyl group analysis of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus. [J] . D L Nuss, E Paoletti Journal of Virology . 1977,第1期

机译：纯化痘苗病毒体外合成病毒型高分子重量RNA的甲基群分析。
3. Cell-free translation of RNA synthesized in vitro by a transcribing nucleoprotein complex prepared from purified vesicular stomatitis virus. [J] . C M Preston, J F Szilagyi Journal of Virology . 1977,第3期

机译：通过纯化的脉湿性口炎病毒制备的转录核蛋白复合物在体外合成的RNA的无细胞翻译。
4. Spectral Graph Partitioning Analysis of In Vitro Synthesized RNA Structural Folding [C] . Stanley NG Kwang Loong, Santosh K. Mishra International Workshop on Pattern Recognition in Bioinformatics(PRIB 2006); 20060820; Hong Kong(CN) . 2006

机译：体外合成的RNA结构折叠的光谱图分区分析
5. NMR analysis of the thymidylate synthase mRNA binding site 1-paromomycin interaction, Moorella thermoacetica selenocysteine elongation factor binding surface for its SECIS RNA, and the nucleocapsid hairpin loop structure of the Ebola virus. [D] . Tavares, Tony. 2008

机译：胸苷酸合酶mRNA结合位点1-巴龙霉素相互作用，Moorella thermoacetica硒代半胱氨酸延伸因子与其SECIS RNA的结合表面以及埃博拉病毒的核衣壳发夹环结构的NMR分析。
6. Methyl group analysis of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus. [O] . D L Nuss, E Paoletti 1977

机译：纯化牛痘病毒体外合成的病毒体相关高分子量RNA的甲基分析。
7. Translation and identification of the mRNA species synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus. [O] . Both, G W, Moyer, S A, Banerjee, A K 1975

机译：水泡性口炎病毒的病毒体相关RNA聚合酶体外合成的mRNA种类的翻译和鉴定。

Methyl group analysis of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus.

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