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首页> 外文期刊>Journal of Virology >Immunological reactivity of antisera to sodium dodecyl sulfate-derived polypeptides of polyoma virions.
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Immunological reactivity of antisera to sodium dodecyl sulfate-derived polypeptides of polyoma virions.

机译:抗血清的免疫反应性与多马病毒素的十二烷基硫酸钠衍生多肽。

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摘要

A study was undertaken to produce antisera to sodium dodecyl sulfate-derived polyoma virion polypeptides. With the use of this antisera, it was possible to detect, by immunofluorescence, cytoplasmic synthesis of V1, V2, and V3 polypeptides at 18 h postinfection and subsequent transport to the nucleus by 22 h postinfection. Anti-V1, anti-V2, and anti-V3 sera did not react with intact virions in an immunodiffusion assay, nor did they possess hemagglutination inhibition or viral neutralization activity. Antiserum produced against the four host histone polypeptides (V4 through V7) demonstrated immunofluorescence when reacted with polyoma-infected cells but not with uninfected cells. Antihistone serum was also capable of neutralizing viral infectivity, inhibiting hemagglutination and reacting with whole virions in an immunodiffusion assay.
机译:进行了一项研究以生产抗血糖钠硫酸钠衍生的多马病毒藻藻藻。通过使用该抗血清,可以通过免疫荧光,v1,v2和v3多肽的v1,v2和v3多肽的检测,并在出现22小时将其运输到细胞核。抗V1,抗V2和抗V3血清没有与免疫气化测定中的完整病毒酮反应,它们也没有具有血液凝集抑制或病毒中和活​​性。与四个宿主组蛋白多肽(V4至V7)产生的抗血清,当与多瘤感染细胞的但没有未感染的细胞时,表明免疫荧光。抗甾酮血清还能够中和病毒感染性,抑制血凝和与整个病毒素中的免疫分子测定中的反应。

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