首页> 美国政府科技报告 >Immunological Studies on Leukocytes: Development of Monospecific Antisera; Cell Specificities of Cytotoxic Iso-Antibodies; Iso-Antigens on Skin Fibroblasts; Preservation of Lymphocytes
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Immunological Studies on Leukocytes: Development of Monospecific Antisera; Cell Specificities of Cytotoxic Iso-Antibodies; Iso-Antigens on Skin Fibroblasts; Preservation of Lymphocytes

机译:白细胞免疫学研究:单特异性抗血清的发展;细胞毒性异抗体的细胞特异性;皮肤成纤维细胞上的异抗原;保存淋巴细胞

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About 400 sera derived mostly from women during or after pregnancy were screened for the presence of leucocyte antibodies with the cytotoxic antibody test. 96 positively reacting sera were found. With some of these sera frequency studies and crossabsorption experiments were performed. Up to now at least six of these sera have given no indication of containing more than one antibody. Studies regarding the difference in cell specificity of the leucocyte antibodies were performed also. It was shown that three kinds of antibodies are demonstrable: antibodies directed against antigens present only on lymphocytes, antibodies directed against antigens present only on granulocytes and antibodies directed against antigens present on both types of cells. Studies on the presence of white cell iso-antigens on skin cells showed that only those antigens that are present both on lymphocytes and granulocytes are demonstrable on fibroblasts grown from skin explants. The preservation of lymphocytes by freezing in liquid nitrogen was studied. Preliminary experiments showed variable results with recoveries between 50 - 95%. After working out a more standardized procedure, the quantitative recovery could be greatly improved. Studies regarding the qualitative recovery are in progress. (Author)

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