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首页> 外文期刊>Journal of Virology >Complexity and polyadenylic acid content of visna virus 60-70S RNA.
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Complexity and polyadenylic acid content of visna virus 60-70S RNA.

机译：visna病毒60-70s RNA的复杂性和聚腺苷酸含量。

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The genomic complexity of visna virus was measured by quantitative analysis of 18 RNase T1-resistant oligonucleotides from 60-70S RNA. T1-resistant oligonucleotides were separated by two-dimensional polyacrylamide gel electrophoresis. Visna virus had a genomic complexity of 3.6 X 10(6) daltons, very close to the size of a single 30-40S RNA subunit. It was therefore concluded that the visna virus genome is largely polyploid. Visna virus 60-70S RNA polyadenylic acid segment was purified by T1 RNase digestion followed by oligodeoxythymidylic acid-cellulose column chromatography. It contained over 99% AMP and had a size of about 200 nucleotides. The binding capacities on oligodeoxythymidylic acid-cellulose of native 60-70S RNA and purified 30-40S RNA subunits were examined. It was concluded that two out of three intact subunits contain a polyadenylic acid segment.
机译：通过从60-70s RNA的18个RNaseT1抗性寡核苷酸的定量分析测量visna病毒的基因组复杂性。通过二维聚丙烯酰胺凝胶电泳分离T1抗性寡核苷酸。 visna病毒的基因组复杂性为3.6×10（6）道尔顿，非常接近单个30-40s RNA亚基的大小。因此，得出结论认为visna病毒基因组在很大程度上是多倍体。 visna病毒60-70s通过T1 RNase消化纯化RNA多腺苷酸区段，然后纯化寡脱羟基酰胺酸 - 纤维素柱色谱。它含有超过99％的放大器，大小约为200个核苷酸。检查了天然60-70s RNA的oligodoxythythydylic酸纤维素的结合能力并纯化了30-40s RNA亚基。结论是，三个完整亚基中的两个含有多腺苷酸区段。

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