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首页> 外文期刊>Journal of Virology >Complementation rescue of Rous sarcoma virus from transformed mammalian cells by polyethylene glycol-mediated cell fusion.
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Complementation rescue of Rous sarcoma virus from transformed mammalian cells by polyethylene glycol-mediated cell fusion.

机译：聚乙二醇介导细胞融合，来自转化哺乳动物细胞的Rous Sarcoma病毒的互补。

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摘要

Polyethylene glycol (PEG) is effective as a fusing agent for the rescue of virus from Rous sarcoma virus-transformed mammalian cells. The procedure of PEG-mediated rescue of virus from virogenic cell lines is described, and the technique is compared with that of Sendai virus-mediated rescue. Virus may be rescued quantitatively from virogenic cell lines by plating mitomycin C-killed transformed mammalian cells with chicken embryo cells, treating the monolayers with 50% PEG and overlaying the monolayers with focus agar. The number of foci that appeared reflected the number of heterokaryons in the fusion mixtures that released infectious virus. PEG gave reproducible results in virus rescue experiments with an efficiency equal to the best Sendai virus preparations. In addition to the description of the technique for PEG-mediated virus rescue from virogenic cell lines, a method for virus rescue from nonvirogenic lines is presented. Preinfection of the chicken embryo cells with helper avian leukosis virus (Rous-associated virus) prior to fusion with mammalian cells transformed by defective viruses complements the virus defect. We examined four nonvirogenic cell lines, and all released infectious virus in the complementation rescue assay.
机译：聚乙二醇（PEG）作为来自肉瘤病毒转化的哺乳动物细胞的病毒拯救的融合剂是有效的。描述了PEG介导的病毒从遗传细胞系拯救的方法，并将该技术与仙台病毒介导的救援进行了比较。通过用鸡胚细胞电镀丝霉素C杀灭转化的哺乳动物细胞，可以从遗传学细胞系中定量地从病毒细胞系中抵押病毒，用50％peg处理单层并用聚焦琼脂覆盖单层。出现的焦点数反映了释放传染病毒的融合混合物中的异质子的数量。 PEG在病毒救援实验中产生可重复的结果，其效率等于最佳仙台病毒制剂。除了从遗传细胞系中拯救的PEG介导的病毒技术的描述之外，还提出了一种用于从非血管素拯救的方法。在用辅助禽血液菌病毒（不相关病毒）与通过缺陷病毒转化的哺乳动物细胞进行融合之前，将鸡胚细胞进行预先食用。我们检查了四种非血管生成细胞系，并在互补救援测定中释放的传染性病毒。

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