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A convenient sequencing method for 5′ protein-linked RNAs

机译：5'蛋白连接的RNA的方便测序方法

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A convenient nucleotide sequencing method for 5′ end protein-linked RNAs was developed. Genome of LSc, 2ab poliovirus, which has a protein (VPg) covalently linked to the 5′ terminus, was labelled with 125I Bolton and Hunter reagent after proteinase K treatment. No sign of labelling of nucleotide moiety in the genome with the reagent was detected. A labelled oligo peptide-linked ribonuclease T1 fragment was obtained from the 5′ end of the genome. Analysis of the complex by two dimensional gel electrophoresis after partial alkali digestion or by the nucleotide sequencing method of Donis-Keller et al. (1) revealed that LSc, 2ab strain genome had an identical 5′ end structure to that of Mahoney strain genome, that is, VPg-pUpUpApApApApCpApGp. Our results have shown that this labelling method is useful for analysis of 5′end sequence of RNAs linked to protein at the 5′ termini.
机译：开发了5'末端蛋白连接的RNA的方便核苷酸测序方法。 LSC的基因组，具有与5'末端共价连接的蛋白质（VPG）的蛋白质（VPG）的基因组用蛋白酶K处理后用 12 5 I Bolton和Hunter试剂标记。没有检测到与试剂的基因组中核苷酸部分标记的标记迹象。标记的寡核苷酸肽连接的核糖核酸酶T1片段是从基因组的5'末端获得的。分析部分碱消解后二维凝胶电泳的复合物分析，或者唐氏甲酮等核苷酸测序方法。 （1）显示LSC，2AB菌株基因组对Mahoney菌株基因组的相同5'末端结构，即VPG-PUPAPAPAPAPAPAPPAPGP。我们的研究结果表明，该标记方法可用于分析5'末端连接到蛋白质的5'end序列。

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