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首页> 外文期刊>Nucleic acids research >Conformation of DNA in chromatin protein-DNA complexes studied by infrared spectroscopy
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Conformation of DNA in chromatin protein-DNA complexes studied by infrared spectroscopy

机译：红外光谱研究染色质蛋白-DNA复合物中DNA的构象

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The following observations concerning the DNA secondary structures in various nucleohistone complexes were made by infrared spectroscopy: 1/ in chromatin, chromatin extracted by 0.6 M NaCl, nucleosomes and histone-DNA reconstituted complexes, the DNA remains in a B type conformation at low relative hygrometry; 2/ in chromatin extracted by tRNA and in non hi stone protein-DNA reconstituted complexes, the DNA can adopt an A type conformation. Infrared linear dichroism data show that in NHP-DNA complexes the low relative hygrometry conformation of DNA may be modified and that the infrared parameter θ1090 is close to that measured for RNA's or DNA-RNA hybrids. It is concluded that the histones block the DNA in a B form and that some of the NHP could be involved in the control of the secondary structure of DNA in chromatin.
机译：通过红外光谱法制造关于各种核酮配合物中DNA二次结构的以下观察：1 /在染色质中，染色质提取0.6M NaCl，核桃瘤和组蛋白-DNA重构复合物，DNA在低相对湿法下保持B型构象; 2 /在TRNA提取的染色质中，在非HITH蛋白-DNA重构复合物中，DNA可以采用型构象。红外线性二色性数据表明，在NHP-DNA复合物中，可以修饰DNA的低相对湿法统一，并且红外参数θ 1090 接近RNA或DNA-RNA杂种的测量。结论是，组蛋白以B形式阻断DNA，并且一些NHP可以参与染色质DNA的二次结构的控制。

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